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hnRNP F Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-46099

Novus Biologicals, part of Bio-Techne
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NBP3-46099

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

heterogeneous nuclear ribonucleoprotein F. (Uniprot# P52597)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit hnRNP F Antibody - BSA Free (NBP3-46099) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for hnRNP F Antibody - BSA Free

hnRNP F Antibody - BSA Free

Immunohistochemistry: hnRNP F Antibody - BSA Free [NBP3-46099] -

Immunohistochemistry: hnRNP F Antibody - BSA Free [NBP3-46099] - Immunohistochemistry of paraffin-embedded human colon cancer using (hnRNP F antibody) at dilution of 1:50
hnRNP F Antibody - BSA Free

Western Blot: hnRNP F Antibody - BSA Free [NBP3-46099] -

Western Blot: hnRNP F Antibody - BSA Free [NBP3-46099] - HeLa cells were subjected to SDS PAGE followed by western blot with (hnRNP F antibody) at dilution of 1:1000

Applications for hnRNP F Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% Glycerol, pH (7.3)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: hnRNP F

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to pre-mRNA processing and transport, and also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP complexes are the major constituents of the spliceosome and in particular, the hnRNP A1 protein is one of the major premRNA/ mRNA binding proteins and also one of the most abundant proteins in the nucleus. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. Most hnRNP proteins, including hnRNP C1 and C2, contain one or more RNA binding domains and are implicated in the processing of pre-mRNA. hnRNPs F and H are largely related factors that preferentially associate with poly(rG) regions on RNA. Isoforms of these proteins are often generated by alternative processing of the premRNA and by posttranslational modifications such as phosphorylation on serines and threonines and methylation of arginines.

Alternate Names

heterogeneous nuclear ribonucleoprotein F, hnRNP F, HNRPFHnRNP F protein, mcs94-1, MGC110997, Nucleolin-like protein mcs94-1, OK/SW-cl.23

Gene Symbol

HNRNPF

Additional hnRNP F Products

Product Documents for hnRNP F Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for hnRNP F Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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