Fibrillin 1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35691
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 360-460 of human Fibrillin 1 (NP_000129.3).
Sequence:
CDAGRCWSPGVTVAPEMCPIRATEDFNKLCSVPMVIPGRPEYPPPPLGPIPPVLPVPPGFPPGPQIPVPRPPVEYLYPSREPPRVLPVNVTDYCQLVRYLC
Sequence:
CDAGRCWSPGVTVAPEMCPIRATEDFNKLCSVPMVIPGRPEYPPPPLGPIPPVLPVPPGFPPGPQIPVPRPPVEYLYPSREPPRVLPVNVTDYCQLVRYLC
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
312 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Fibrillin 1 Antibody - BSA Free
Immunohistochemistry: Fibrillin 1 Antibody [NBP3-35691] -
Immunohistochemistry: Fibrillin 1 Antibody [NBP3-35691] - Immunohistochemistry analysis of paraffin-embedded Human testis using Fibrillin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunocytochemistry/ Immunofluorescence: Fibrillin 1 Antibody [NBP3-35691] -
Immunocytochemistry/ Immunofluorescence: Fibrillin 1 Antibody [NBP3-35691] - Immunofluorescence analysis of NIH-3T3 cells using Fibrillin 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western Blot: Fibrillin 1 Antibody [NBP3-35691] -
Western Blot: Fibrillin 1 Antibody [NBP3-35691] - Western blot analysis of lysates from Rat pancreas, using Fibrillin 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Applications for Fibrillin 1 Antibody - BSA Free
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Fibrillin-1/FBN1
Long Name
Fibrillin-1
Alternate Names
ACMICD, Asprosin, Fibrillin 1, GPHYSD2, SSKS, WMS2
Gene Symbol
FBN1
Additional Fibrillin-1/FBN1 Products
Product Documents for Fibrillin 1 Antibody - BSA Free
Product Specific Notices for Fibrillin 1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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