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FE65 Antibody (23GB3665)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51125

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NBP3-51125-100ul
NBP3-51125-20ul

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB3665

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human FE65 (Uniprot: O00213)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FE65 Antibody (23GB3665)

FE65 Antibody (23GB3665)

Immunocytochemistry/ Immunofluorescence: FE65 Antibody (23GB3665)[NBP3-51125] -

Immunocytochemical staining of HAP-1 cells with FE65 antibody (1:1,000). Nuclei were stained blue with DAPI; FE65 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 um.
FE65 Antibody (23GB3665)

Flow Cytometry: FE65 Antibody (23GB3665)[NBP3-51125] -

Validation of FE65 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with FE65 antibody (1:2,000) and analyzed using BD flow cytometer.
FE65 Antibody (23GB3665)

Flow Cytometry: FE65 Antibody (23GB3665)[NBP3-51125] -

Flow cytometric analysis of FE65expression in HAP-1 cells using FE65 antibody (1:2,000). Green, isotype control; red, APBB1.

Applications for FE65 Antibody (23GB3665)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FE65

Amyloid beta A4 precursor protein-binding family B member 1, also known as FE65, is a signaling adaptor of APP protein. The FE65/APP complex has been shown to translocate into the cell nucleus and regulate gene expression, indicating that FE65 may be critical in the pathogenesis of Alzheimer's disease. Genetic studies in mice validated FE65-APP interaction and molecular studies provided evidence on the role of the complex in abnormal signaling detected in brains of Alzheimer's Disease patients.

FE65 antibodies are useful tools for research on Alzheimer's disease progression.

Alternate Names

adaptor protein FE65a2, amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65), amyloid beta A4 precursor protein-binding, family B, member 1, Fe65, FE65amyloid beta A4 precursor protein-binding family B member 1, MGC:9072, Protein Fe65, RIR, stat-like protein

Gene Symbol

APBB1

Additional FE65 Products

Product Documents for FE65 Antibody (23GB3665)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FE65 Antibody (23GB3665)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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