FCRN/FCGRT Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89128

Novus Biologicals, part of Bio-Techne
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NBP1-89128
NBP1-89128-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Cynomolgus Monkey

Cited:

Human, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Simple Western

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FCRN Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LTFLLFSCPHRLREHLERGRGNLEWKEPPSMRLKARPSSPGFSVLTCSAFSFYPPELQLRFLRNGLAAGTGQGDFGPNSDGSFHASSSLTVKSGDEHHYCCIVQHAGLAQPLRVELESPAKS

Reactivity Notes

Cynomolgus Monkey reactivity reported in scientific literature (PMID: 31746560).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Knockout (KO) Validated Rabbit FCRN/FCGRT Antibody - BSA Free (NBP1-89128) is a polyclonal antibody validated for use in IHC, WB and Simple Western. Anti-FCRN/FCGRT Antibody: Cited in 15 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FCRN/FCGRT Antibody - BSA Free

Knockout Validated: FCRN/FCGRT Antibody [NBP1-89128]

Western Blot: FCRN/FCGRT Antibody [NBP1-89128]

Western Blot: FCRN/FCGRT Antibody [NBP1-89128] - Western blot shows lysates of 293 human embryonic kidney parental cell line and FCGRT knockout (KO) 293 cell line. PVDF membrane was probed with 1:500 of Rabbit Anti-Human FCGRT Polyclonal Antibody (Catalog # NBP1-89128) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FCGRT at approximately 40 kDa (as indicated) in the parental 293 cell line, but is not detectable in the knockout 293 cell line. This experiment was conducted under reducing conditions.
Western Blot: FCRN/FCGRT Antibody [NBP1-89128]

Western Blot: FCRN/FCGRT Antibody [NBP1-89128]

Western Blot: FCRN/FCGRT Antibody [NBP1-89128] - Analysis in human cell lines Caco-2 and HeLa using anti-FCGRT antibody. Corresponding FCGRT RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Western Blot: FCRN/FCGRT Antibody [NBP1-89128]

Western Blot: FCRN/FCGRT Antibody [NBP1-89128]

FCRN-FCGRT-Antibody-Western-Blot-NBP1-89128-img0029.jpg
View 10 more images

Applications for FCRN/FCGRT Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Frozen

Reported in scientific literature (PMID 24278022).

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Simple Western

1:20

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in h. Liver, HepG2 (middle) and THP-1, separated by Size, antibody dilution of 1:20, apparent MW was 51, 44 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-89128 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FCRN

The FCGRT gene encodes a 365 amino acid long, 39 kDA IgG receptor FcRn large subunit p51 protein that transfers immunoglobulin G antibodies across the placenta from mother to fetus, as well as functions to protect the antibody from degradation. FCGRT interacts with genes CA6, ELANE, AZGP1, B2M, and ALB. It has been linked to diseases such as: lupus, nephritis, leukemia, arthritis, immunodeficiency, epidermolysis bullosa acquisita, persistent fetal circulation syndrome, and pemphigus.

Long Name

Neonatal Fc Receptor

Alternate Names

FCGRT

Entrez Gene IDs

2217 (Human)

Gene Symbol

FCGRT

UniProt

P55899

Additional FCRN Products

Product Documents for FCRN/FCGRT Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FCRN/FCGRT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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