FAAH Antibody (10A1M7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16330
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10A1M7 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 480-579 of human FAAH (O00519). DLNAPGRATGAVSYTMLYNCLDFPAGVVPVTTVTAEDEAQMEHYRGYFGDIWDKMLQKGMKKSVGLPVAVQCVALPWQEELCLRFMREVERLMTPEKQSS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit FAAH Antibody (10A1M7) (NBP3-16330) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for FAAH Antibody (10A1M7)
![Western Blot: FAAH Antibody (10A1M7) [NBP3-16330]](https://resources.bio-techne.com/images/products/FAAH-Antibody-10A1M7-Western-Blot-NBP3-16330-img0003.jpg "Western Blot: FAAH Antibody (10A1M7) [NBP3-16330]")
Western Blot: FAAH Antibody (10A1M7) [NBP3-16330]
Western Blot: FAAH Antibody (10A1M7) [NBP3-16330] - Western blot analysis of extracts of various cell lines, using FAAH Rabbit mAb (NBP3-16330) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: FAAH Antibody (10A1M7) [NBP3-16330] -
Immunocytochemistry/ Immunofluorescence: FAAH Antibody (10A1M7) [NBP3-16330] - Confocal imaging of NIH/3T3 cells using FAAH Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
FAAH Antibody (10A1M7) [NBP3-16330] -
Analysis of various lysates using FAAH1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.Applications for FAAH Antibody (10A1M7)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL.
Immunocytochemistry/ Immunofluorescence
1:200 - 1:800
Western Blot
1:1000 - 1:4000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FAAH
Long Name
Fatty Acid Amide Hydrolase
Alternate Names
FAAH-1, FAAH1, PSAB
Gene Symbol
FAAH
Additional FAAH Products
Product Documents for FAAH Antibody (10A1M7)
Product Specific Notices for FAAH Antibody (10A1M7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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