epithelial Sodium Channel gamma Antibody (3c7) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41371
![Western Blot: epithelial Sodium Channel gamma Antibody (3c7)BSA Free [NBP2-41371]](https://resources.bio-techne.com/images/products/epithelial-Sodium-Channel-gamma-Antibody-3c7-BSA-Free-Western-Blot-NBP2-41371-img0006.jpg "Western Blot: epithelial Sodium Channel gamma Antibody (3c7)BSA Free [NBP2-41371]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Sandwich ELISA, Sandwich ELISA Capture
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3c7
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The inhibitory peptide from the human gamma-ENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human gamma-ENaC subunit.
Epitope
The epitope is on the inhibitory tract of human ENaC.
Specificity
Antibody is specific for the inhibitory tract of human gamma-ENaC subunit.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Novus Biologicals Mouse epithelial Sodium Channel gamma Antibody (3c7) - BSA Free (NBP2-41371) is a monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for epithelial Sodium Channel gamma Antibody (3c7) - BSA Free
Western Blot: epithelial Sodium Channel gamma Antibody (3c7)BSA Free [NBP2-41371]
Western Blot: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371] - Analysis on a lysate of HEK293 cells transfected with and without yENaC. Lane 1 loaded with 5ug Lane 2 loaded with 10ug Lane 3 loaded with 20ug![ELISA: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371]](https://resources.bio-techne.com/images/products/epithelial-Sodium-Channel-gamma-Antibody-3c7-BSA-Free-ELISA-NBP2-41371-img0007.jpg "ELISA: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371]")
ELISA: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371]
ELISA: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371] - The calibration curve of a sandwich assay for Anti-gENaC using NBP2-41371 as the capture antibody and NBP2-41373B as the biotinylated detection antibody.Applications for epithelial Sodium Channel gamma Antibody (3c7) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Sandwich ELISA Capture
1-4 ug/ml
Western Blot
1:100-1:2000
Application Notes
ELISA: NBP2-41371 was used in ELISA. A sandwich ELISA can be made using NBP2-41371 (1-4 ug/ml) as the capture antibody and biotinylated NBP2-41373 (0.05 - 0.2 ug/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131). WB: NBP2-41371 was used in Western blot. (1,2) IHC: NBP2-41371 was used in immunohistochemistry.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM Phosphate (pH 7.4) and 0.5M NaCl
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: epithelial Sodium Channel gamma
Long Name
Amiloride-sensitive sodium channel subunit gamma
Alternate Names
ENaCG, Gamma-ENaC, Gamma-NaCH, SCNEG, SCNN1G
Gene Symbol
SCNN1G
Additional epithelial Sodium Channel gamma Products
Product Documents for epithelial Sodium Channel gamma Antibody (3c7) - BSA Free
Product Specific Notices for epithelial Sodium Channel gamma Antibody (3c7) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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