DARS Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00268
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human DARS (Accession#: P14868; Met1-Pro501)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for DARS Antibody - Azide and BSA Free
Western Blot: DARS Antibody [NBP3-00268] -
Western Blot: DARS Antibody [NBP3-00268] - NBP3-00268 at 1:500 dilution Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:57 kDa Observed band size:55 kDa (We are unsure as to the identity of these extra bands.)
Immunoprecipitation: DARS Antibody [NBP3-00268] -
Immunoprecipitation: DARS Antibody [NBP3-00268] - DARS was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 2 uL NBP3-00268 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00268, at 1:100 dilution. Secondary antibody: IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDa Observed band size: 55 kDa
Immunohistochemistry-Paraffin: DARS Antibody [NBP3-00268] -
Immunohistochemistry-Paraffin: DARS Antibody [NBP3-00268] - Immunochemical staining of human DARS in human brain with NBP3-00268 (1:10000, formalin-fixed paraffin embedded sections).Applications for DARS Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunohistochemistry-Paraffin
1:2500-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DARS
Alternate Names
aspartate tRNA ligase 1, cytoplasmic, Aspartate--tRNA ligase, aspartyl-tRNA synthetase, aspartyl-tRNA synthetase, cytoplasmic, AspRS, Cell proliferation-inducing gene 40 protein, cell proliferation-inducing protein 40, DKFZp781B11202, EC 6.1.1.12, MGC111579
Gene Symbol
DARS1
Additional DARS Products
Product Documents for DARS Antibody - Azide and BSA Free
Product Specific Notices for DARS Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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