CTCF Antibody (23GB3520)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51100
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB3520
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human CTCF (Uniprot: P49711)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CTCF Antibody (23GB3520)
Immunocytochemistry/ Immunofluorescence: CTCF Antibody (23GB3520)[NBP3-51100] -
CTCF antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: CTCF shRNA knockdown (KD). Nuclei were stained blue with DAPI; CTCF was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
Flow Cytometry: CTCF Antibody (23GB3520)[NBP3-51100] -
Validation of CTCF knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with CTCF antibody (1:2,000) and analyzed using BD flow cytometer.
Flow Cytometry: CTCF Antibody (23GB3520)[NBP3-51100] -
Flow cytometric analysis of CTCFexpression in C2C12 cells using CTCF antibody (1:2,000). Green, isotype control; red, CTCF.Applications for CTCF Antibody (23GB3520)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CTCF
Long Name
CCCTC-binding Factor
Alternate Names
11-Zinc Finger Protein2, CCCTC-binding Factor
Gene Symbol
CTCF
Additional CTCF Products
Product Documents for CTCF Antibody (23GB3520)
Product Specific Notices for CTCF Antibody (23GB3520)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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