CRISPR-Cpf1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62735

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Bacterial

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Cpf1 Antibodies »

Product Specifications

Immunogen

Partial recombinant Francisella tularensis protein (between amino acids 600-850) [NCBI WP_003034647.1]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CRISPR-Cpf1 Antibody - BSA Free

Western Blot: CRISPR-Cpf1 AntibodyBSA Free [NBP2-62735]

Western Blot: CRISPR-Cpf1 AntibodyBSA Free [NBP2-62735]

Western Blot: CRISPR-Cpf1 Antibody [NBP2-62735] - Partial recombinant Cpf1 protein (Ca. 12 kDa) was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Cpf1 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Lane 1, 10 ng, Lane 2. 5 ng.

Applications for CRISPR-Cpf1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cpf1

Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) is an adaptive immune system in bacteria which provides protection against mobile genetic elements from viruses such as, transposable elements and conjugative plasmids. CRISPR clusters are known to have sequences which are complementary to antecedent mobile elements and target invading nucleic acids, and these sequences are transcribed/processed into CRISPR RNA. CRISPR possess endonuclease activity on pre-crRNA and dsDNA, using different active sites, and in a complexed form, crRNA displaces the non-target DNA strand and guides endo-nucleolytic cleavage of both strands of the DNA. CRISPR associated Cas12a (CRISPR-Cas12a), also known as Cpf1, is emerging as a genome editing tool with different target specificity, when compared to CRISPR-Cas9. CRISPR-Cas12a-assisted recombineering can be used for genetic manipulation of plasmids, plasmid curing and for a more precise selection of target sites for the introduction of desired mutations (point mutations, deletions, insertions, and replacements). In cells subjected to genetic manipulation using this tool, CRISPR-Cas12a antibodies are needed to verify transfection efficiency, CRISPR-Cas12a expression, CRISPR-Cas12a target binding and subcellular localization. Novus Biologicals' CRISPR-Cpf1 antibody (NBP2-62735) is the first-ever commercially launched antibody to meet this need. This antibody is generated against a partial recombinant Francisella tularensis protein (between amino acids 600-850) [NCBI WP_003034647.1], and validated for Western blot using a partial recombinant CRISPR-Cpf1 protein (12kDa), making it a great tool for a direct detection of CRISPR-Cpf1/CRISPR-Cas12a complex (at protein level) superior to the use of epitope-tag based validation approaches.

Long Name

CRISPR-associated Endonuclease Cas12a

Alternate Names

Cas12a, anti-cas12a, anti-cas12a antibody, anti-CPF1, anti-CRISPR-Cas12a, anti-CRISPR-Cpf1

UniProt

A0Q7Q2 (Bacteria)

Additional Cpf1 Products

Product Documents for CRISPR-Cpf1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CRISPR-Cpf1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.