CD8 beta Antibody (H35-17.2) [Allophycocyanin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08222APC
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse, Human (Negative)
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2b Kappa Clone # H35-17.2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c (Uniprot: P10300)
Reactivity Notes
Does not react with Human.
Localization
Cell Surface and Secreted
Specificity
This monoclonal antibody reacts with the Mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. This monoclonal antibody identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2b Kappa
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Applications for CD8 beta Antibody (H35-17.2) [Allophycocyanin]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP3-08222. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
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Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: CD8 beta
Alternate Names
CD8 beta, CD8b, CD8B1, Leu2, LY3, LYT3, P37
Gene Symbol
CD8B
Additional CD8 beta Products
Product Documents for CD8 beta Antibody (H35-17.2) [Allophycocyanin]
Product Specific Notices for CD8 beta Antibody (H35-17.2) [Allophycocyanin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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