Cathepsin X/Z/P Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38614

Novus Biologicals, part of Bio-Techne
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NBP2-38614
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cathepsin X/Z/P Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PDETCNNYQAKDQECDKFNQCGTCNEFKECHAIRNYTLWRVGDYGSLSGREKMMAEIYANGPISCGIMATERLANYTGGIYAEYQD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cathepsin X/Z/P Antibody - BSA Free

Western Blot: Cathepsin X/Z/P Antibody [NBP2-38614]

Western Blot: Cathepsin X/Z/P Antibody [NBP2-38614]

Western Blot: Cathepsin X/Z/P Antibody [NBP2-38614] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line CACO-2
Immunocytochemistry/ Immunofluorescence: Cathepsin X/Z/P Antibody [NBP2-38614]

Immunocytochemistry/ Immunofluorescence: Cathepsin X/Z/P Antibody [NBP2-38614]

Immunocytochemistry/Immunofluorescence: Cathepsin X/Z/P Antibody [NBP2-38614] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Cathepsin X/Z/P Antibody [NBP2-38614]

Immunohistochemistry-Paraffin: Cathepsin X/Z/P Antibody [NBP2-38614]

Immunohistochemistry-Paraffin: Cathepsin X/Z/P Antibody [NBP2-38614] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
View 2 more images

Applications for Cathepsin X/Z/P Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

1:10

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
See Simple Western Antibody Database for Simple Western validation: Tested in SW480 lysates, separated by Size, antibody dilution of 1:10

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cathepsin X/Z/P

Cathepsin X, also known as Cathepsin Z and Cathepsin P, is a relatively new cysteine protease of the papain family. Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue.

Alternate Names

Cathepsin P, Cathepsin Z, CTSZ

Gene Symbol

CTSZ

UniProt

Q9UBR2

Additional Cathepsin X/Z/P Products

Product Documents for Cathepsin X/Z/P Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cathepsin X/Z/P Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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