Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Janelia Fluor 549

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cathepsin A/Lysosomal Carboxypeptidase A Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cathepsin A/Lysosomal Carboxypeptidase A
Ala29-Tyr480
Accession # P10619

Specificity

Detects both pro and active forms of human and mouse Cathepsin A/Lysosomal Carboxypeptidase A in direct ELISAs and Western blots. In Western blots, it recognizes all forms of recombinant human Cathepsin A: single chain (55 kDa), heavy chain (32 kDa), and light chain (20 kDa). Also in Western blots, less than 1% cross-reactivity with recombinant human (rh) Cathepsin B, rhCathepsin C, rhCathepsin D, rhCathepsin L, rhCathepsin L2/V, rhCathepsin O, rhCathepsin S and rhCathepsin X/Z/P is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Cathepsin A/Lysosomal Carboxypeptidase A

Lysosomal protective protein/cathepsin A (PPCA) is a lysosomal serine carboxypeptidase that forms an intralysosomal enzyme-complex with b-galactosidase and neuraminidase (NEU1). PPCA is synthesized as a 54-kDa precursor/zymogen, and proteolytically cleaved in the lysosome into a catalytically active 32- and 20-kDa two-chain enzyme. The enzyme has cathepsin A activity at acidic pH but maintains also a deamidase/esterase activity at neutral pH. Furthermore, the human enzyme, purified from platelets and lymphocytes, has been shown to function on the inactivation of selected neuropeptides, like substance P, oxytocin, and endothelin I. The autosomal recessive genetic deficiency of PPCA causes galactosialidosis, a neurodegenerative lysosomal storage disorder, resulting in the secondary deficiencies of b-galactosidase and NEU1.

Alternate Names

CTSA, Lysosomal Carboxypeptidase A

Gene Symbol

CTSA

Additional Cathepsin A/Lysosomal Carboxypeptidase A Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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