c-jun Antibody (CL9662) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44268

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL9662

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all c-Jun Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P05412, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for c-jun Antibody (CL9662) - Azide and BSA Free

c-jun Antibody (CL9662) - Azide and BSA Free Immunohistochemistry-Paraffin: c-jun Antibody [NBP3-44268]

Immunohistochemistry-Paraffin: c-jun Antibody [NBP3-44268]

Staining of human endometrium shows strong nuclear positivity in glandular cells.
c-jun Antibody (CL9662) - Azide and BSA Free Immunohistochemistry-Paraffin: c-jun Antibody [NBP3-44268]

Immunohistochemistry-Paraffin: c-jun Antibody [NBP3-44268]

Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.
c-jun Antibody (CL9662) - Azide and BSA Free Immunohistochemistry-Paraffin: c-jun Antibody [NBP3-44268]

Immunohistochemistry-Paraffin: c-jun Antibody [NBP3-44268]

Staining of human prostate shows moderate nuclear positivity in glandular cells.
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Applications for c-jun Antibody (CL9662) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: c-Jun

The human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. cJun (previously known as the Fos binding protein p39) and c Fos form a complex in the nucleus. AP 1 (activating protein 1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32 to p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Long Name

Cellular Repressor of E1A-stimulated Genes/Transcription Factor AP-1

Alternate Names

cJun, JUN

Gene Symbol

JUN

Additional c-Jun Products

Product Documents for c-jun Antibody (CL9662) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for c-jun Antibody (CL9662) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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