BARD1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13065
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human BARD1. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for BARD1 Antibody - BSA Free
![Immunohistochemistry-Paraffin: BARD1 Antibody [NBP3-13065]](https://resources.bio-techne.com/images/products/BARD1-Antibody-Immunohistochemistry-Paraffin-NBP3-13065-img0005.jpg "Immunohistochemistry-Paraffin: BARD1 Antibody [NBP3-13065]")
Immunohistochemistry-Paraffin: BARD1 Antibody [NBP3-13065]
Immunohistochemistry-Paraffin: BARD1 Antibody [NBP3-13065] - BARD1 antibody detects BARD1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. BARD1 stained by BARD1 antibody (NBP3-13065) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: BARD1 Antibody [NBP3-13065] -
Western Blot: BARD1 Antibody [NBP3-13065] - A431 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BARD1 antibody (NBP3-13065) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: BARD1 Antibody [NBP3-13065] -
BARD1 antibody detects BARD1 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BARD1 protein stained by BARD1 antibody (NBP3-13065) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
Applications for BARD1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BARD1
Long Name
BRCA1 associated RING domain 1
Alternate Names
BARD-1, BRCA1 associated RING domain 1, BRCA1-associated RING domain gene 1, BRCA1-associated RING domain protein 1, EC 6.3.2.-
Gene Symbol
BARD1
Additional BARD1 Products
Product Specific Notices for BARD1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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