BARD1 Antibody - BSA Free

Western Blot: BARD1 Antibody [NB100-319]

Western Blot: BARD1 Antibody [NB100-319] - Detection of human BARD1 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, Hep-G2, K-562, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BARD1 antibody NB100-319 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: BARD1 Antibody [NB100-319]

Immunoprecipitation: BARD1 Antibody [NB100-319] - Detection of human BARD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BARD1 antibody NB100-319 (NB100-319 lot 4) used for IP at 6 ug per reaction. BARD1 was also immunoprecipitated by a previous lot of this antibody (NB100-319 lot 3). For blotting immunoprecipitated BARD1, NB100-319 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Western Blot: BARD1 Antibody - BSA Free [NB100-319] -

MitoT increases the expression of anti-apoptotic Bcl-2/BARD1 pathways. A Fold change of differentially expressed (DE) genes contained within the GO terms related to apoptosis, cell death, and/or responses to different stimuli. B qRT-PCR analysis of BARD1 mRNA expression levels in FACS-sorted CD3+ T cells after 24-h post-mitoception with MSC derived-MT (n = 4). C Representative western blots of BARD1 in FACS-sorted CD3+ MitoTpos cells and MitoTneg cells after 24 or 48 h post-mitoception. D Fold change quantification of proteins showed in C (n = 3). E Ingenuity Pathway analysis for Bcl-2 gene network for the DE genes denoted in A. F qRT-PCR of Bcl-2 family gene expression in FACS-sorted CD3+ MitoTpos cells and MitoTneg cells after 24 h post-mitoception (n = 4). For figures B and D: graphs show mean +/- SEM and statistical analysis by unpaired t-test. For figure F: graph shows mean +/- SEM and statistical analysis by unpaired Mann–Whitney test (*p < 0.05, relative to MitoTneg control). All replicates are biological Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39334383), licensed under a CC-BY license. Not internally tested by Novus Biologicals.