BAF53A Antibody (23GB1155)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51997

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NBP3-51997-100ul
NBP3-51997-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB1155

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BAF53A Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human BAF53A (Uniprot: O96019)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BAF53A Antibody (23GB1155)

BAF53A Antibody (23GB1155)

Immunocytochemistry/ Immunofluorescence: BAF53A Antibody (23GB1155)[NBP3-51997] -

Immunocytochemical staining of HT-1080 cells with BAF53A antibody (1:1,000). Nuclei were stained blue with DAPI; BAF53A was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
BAF53A Antibody (23GB1155)

Flow Cytometry: BAF53A Antibody (23GB1155)[NBP3-51997] -

Flow cytometric analysis of BAF53Aexpression in HepG2 cells using BAF53A antibody (1:2,000). Green, isotype control; red, Actin like 6A.
BAF53A Antibody (23GB1155)

Western Blot: BAF53A Antibody (23GB1155)[NBP3-51997] -

Western blotting analysis using BAF53A antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for BAF53A Antibody (23GB1155)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BAF53A

BAF53A encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.

Alternate Names

actin-like 6A, actin-like protein 6A, actin-related protein 4,53 kDa BRG1-associated factor A, Actin-related protein Baf53a, Actl6, Arp4, ArpNbeta, BAF complex 53 kDa subunit, Baf53a, BAF53AACTL6, BAF53MGC5382, BRG1-associated factor 53A, hArpN beta, INO80 complex subunit K, INO80KARPN-BETA

Gene Symbol

ACTL6A

Additional BAF53A Products

Product Documents for BAF53A Antibody (23GB1155)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BAF53A Antibody (23GB1155)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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