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Aromatase Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1596

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Primate, Rabbit

Cited:

Human, Mouse, Rat, Primate, Rabbit

Predicted:

Bovine (90%), Equine (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the human Aromatase protein (between residues 400-502). [UniProt# P11511]

Reactivity Notes

Use in Rabbit reported in scientific literature (PMID:35245530). Rabbit reactivity reported in scientific literature (PMID: 25850953). Immunogen displays the following percentage of sequence identity for non-tested species: horse (91%), cow (90%), pig (83%), sheep (81%), goat (81%), and dog (81%) proteins. Rat reactivity reported in scientific literature (PMID: 29898754). Use in Mouse reported in scientific literature (PMID:32634520).

Localization

Membrane-bound

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aromatase Antibody - BSA Free

Western Blot: Aromatase Antibody [NB100-1596]

Western Blot: Aromatase Antibody [NB100-1596]

Aromatase-Antibody-Western-Blot-NB100-1596-img0005.jpg
Immunocytochemistry/ Immunofluorescence: Aromatase Antibody [NB100-1596]

Immunocytochemistry/ Immunofluorescence: Aromatase Antibody [NB100-1596]

Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NB100-1596] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aromatase Antibody NB100-1596 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596]

Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596]

Aromatase-Antibody-Immunohistochemistry-Paraffin-NB100-1596-img0006.jpg

Applications for Aromatase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200. Use reported in scientific literature (PMID 28133606)

Immunohistochemistry-Paraffin

1:200

Simple Western

reported in scientific literature (PMID 29898754)

Western Blot

1 ug/ml
Application Notes
In Western blot, a band is seen at ~55 kDa in human brain, and Immunocytochemistry/Immunofluorescence where membrane staining is observed in SH-SY-5Y cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aromatase

Aromatase (ARO1) or cytochrome P450 19A1 (CYP19A1) belongs to cytochrome P450 family of enzymes, which are monooxygenases known for their essential role in drug catabolism and cholesterol/steroid synthesis. Employing heme-group act as a co-factor, ARO1 catalyzes aromatic C18 estrogens formation from C19 androgens and is responsible for conversion of testosterone to estradiol and androstenedione to estrone. ARO1 is localized to cellular membranes as a peripheral membrane protein and in addition to female reproductive tract and adipose tissue, aromatase can be found in testis, liver, brain as well as certain tumors including breast cancer. During ontogenesis, it plays an important role in the development of CNS and gonads as well as sex differentiation. Defective ARO1 may leads to aromatase excess syndrome (AEXS) and aromatase deficiency (AROD). Moreover, obesity has been linked to abnormally high ARO1 expression in mammary tissues, increased local estrogen production, and susceptibility to breast hyperplasia and cancer. ARO1 is the key enzyme in intratumoral estrogen production in postmenopausal mammary cancer.

Long Name

Aromatase

Alternate Names

ARO1, CYAR, CYP19, CYP19A1, CYPXIX, EC 1.14.14.14, Estrogen synthase

Gene Symbol

CYP19A1

Additional Aromatase Products

Product Documents for Aromatase Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aromatase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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