Aquaporin-8 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92557
![Immunocytochemistry/ Immunofluorescence: Aquaporin-8 Antibody - BSA Free [NBP2-92557]](https://resources.bio-techne.com/images/products/Aquaporin-8-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92557-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Aquaporin-8 Antibody - BSA Free [NBP2-92557]")
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Aquaporin-8 (NP_001160.2). MLIGGLNLVMLLPYWVSQLLGGMLGAALAKAVSPEERFWNASGAAFVTVQEQGQVAGALVAEIILTTLLALAVCMGAINEKTKGPLAPFSIGFAVTVDILA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Aquaporin-8 Antibody - BSA Free (NBP2-92557) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Aquaporin-8 Antibody - BSA Free
Western Blot: Aquaporin-8 Antibody - BSA Free [NBP2-92557] -
Western Blot: Aquaporin-8 Antibody - BSA Free [NBP2-92557] - Western blot analysis of various lysates using Aquaporin-8 Rabbit pAb at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates / proteins: 25 ug per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection:ECL Basic Kit.
Exposuretime: 90s.
Western Blot: Aquaporin-8 Antibody - BSA Free [NBP2-92557] -
Western Blot: Aquaporin-8 Antibody - BSA Free [NBP2-92557] - Western blot analysis of lysates from Rat liver, using Aquaporin-8 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: Aquaporin-8 Antibody - BSA Free [NBP2-92557] -
Immunocytochemistry/ Immunofluorescence: Aquaporin-8 Antibody - BSA Free [NBP2-92557] - Immunofluorescence analysis of NIH/3T3 cells using Aquaporin-8 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Applications for Aquaporin-8 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Aquaporin-8
Alternate Names
AQP-8, aquaporin 8, aquaporin-8
Gene Symbol
AQP8
Additional Aquaporin-8 Products
Product Documents for Aquaporin-8 Antibody - BSA Free
Product Specific Notices for Aquaporin-8 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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