Anti- Brentuximab (Anti-Idiotype) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9584
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Multi-Species
Applications
Western Blot, ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2262C
Product Specifications
Immunogen
Full length Brentuximab
Specificity
Detects Brentuximab in Western blots and direct ELISAs. In direct ELISAs, no cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Trastuzumab, Tositumomab, Adalimumab, Gemtuzumab, Solanezumab, Teprotumumab, and Aducanumab is observed.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Anti- Brentuximab (Anti-Idiotype) Antibody
Detection of Brentuximab by Western Blot.
Western blot shows Centuximab (negative control), Rituximab (negative control), Brentuximab, and Gemtuzumab (negative control) biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti-Brentuximab (Idiotype) Monoclonal Antibody (Catalog # MAB9584) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Brentuximab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of anti-CD30/TNFRSF8 (Brentuximab) on Jurkat Human cell line by flow cytometry.
Jurkat human cell line was stained with anti-CD30/TNFRSF8 (Brentuximab, Catalog # MAB9576) then Anti-Brentuximab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9584, filled histogram) or normal rabbit IgG isotype control antibody (MAB1050, open histogram), followed by PE-conjugated anti-rabbit IgG secondary antibody (F0110).
Specificity of Anti-Brentuximab (Anti-Idiotype) Antibody in ELISA Capture Assay.
Biotinylated biosimilars were serially diluted and incubated with Anti-Brentuximab (Anti-Idiotype) Antibody (Catalog # MAB9584, green line) coated at 1 μg/mL on a 96-well polystyrene plate. No cross-reactivity with Rituximab (black line) or biotinylated IgG control (orange line) was observed.Applications for Anti- Brentuximab (Anti-Idiotype) Antibody
Application
Recommended Usage
ELISA
Detects Brentuximab in direct ELISAs.
Flow Cytometry
0.25 µg/106 cells
Sample: Jurkat Human cell line plus anti-CD30/TNFRSF8 (Brentuximab), Catalog # MAB9576
Sample: Jurkat Human cell line plus anti-CD30/TNFRSF8 (Brentuximab), Catalog # MAB9576
Western Blot
0.5 µg/mL
Sample: Brentuximab under non-reducing conditions only
Sample: Brentuximab under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Brentuximab
Additional Brentuximab Products
Product Documents for Anti- Brentuximab (Anti-Idiotype) Antibody
Product Specific Notices for Anti- Brentuximab (Anti-Idiotype) Antibody
For research use only
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