ADK Antibody (23GB625)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52002

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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB625

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ADK Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human ADK (Uniprot: P55263)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ADK Antibody (23GB625)

ADK Antibody (23GB625)

Immunocytochemistry/ Immunofluorescence: ADK Antibody (23GB625)[NBP3-52002] -

Immunocytochemical staining of HAP-1 cells with ADK antibody (1:1,000). Nuclei were stained blue with DAPI; ADK was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
ADK Antibody (23GB625)

Western Blot: ADK Antibody (23GB625)[NBP3-52002] -

Western blotting analysis using ADK antibody. ADKexpression in wild type (WT) and ADKshRNA knockdown (KD) HeLa cells with 30 ug of total cell lysates. Hsp90 alpha serves as a loading control. The blot was incubated with ADK antibody(1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:50,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for ADK Antibody (23GB625)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ADK

Adenosine kinase (ATP:adenosine 5 prime phosphotransferase) is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma phosphate from ATP to adenosine, thereby serving as a potentially important regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of ADK could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as antiinflammatory agents. The encoded protein does not present any sequence similarities to other well characterized mammalian nucleoside kinases. In contrast, 2 regions were identified with significant sequence identity to microbial ribokinase and fructokinases and a bacterial inosine/guanosine kinase. Thus, ADK is a structurally distinct mammalian nucleoside kinase that appears to be akin to sugar kinases of microbial origin.

Long Name

Adenosine Kinase

Alternate Names

AK

Gene Symbol

ADK

Additional ADK Products

Product Documents for ADK Antibody (23GB625)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ADK Antibody (23GB625)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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