ACAT1 Antibody (23GB1255)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51993
Key Product Details
Species Reactivity
Human, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB1255
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human ACAT1 (Uniprot: P24752)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ACAT1 Antibody (23GB1255)
Immunocytochemistry/ Immunofluorescence: ACAT1 Antibody (23GB1255)[NBP3-51993] -
Immunocytochemical staining of HT-1080 cells with ACAT1 antibody (1:1,000) . Nuclei were stained blue with DAPI;ACAT1 with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: ACAT1 Antibody (23GB1255)[NBP3-51993] -
Validation of ACAT1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ACAT1 antibody (1:2,000) and analyzed using BD flow cytometer.
Flow Cytometry: ACAT1 Antibody (23GB1255)[NBP3-51993] -
Flow cytometric analysis of ACAT1expression in HepG2 cells using ACAT1 antibody (1:2,000). Green, isotype control; red, Acetyl-CoA acetyltransferase 1.Applications for ACAT1 Antibody (23GB1255)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ACAT1
Mutations in this gene have been associated with 3-ketothiolase deficiency, an inborn defect of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
Long Name
Acetyl-CoA Acetyltransferase 1
Alternate Names
THIL
Gene Symbol
ACAT1
Additional ACAT1 Products
Product Documents for ACAT1 Antibody (23GB1255)
Product Specific Notices for ACAT1 Antibody (23GB1255)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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