AARSD1 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00270
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human AARSD1 (Uniprot#: Q9BTE6-2; Thr176-Glu525).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for AARSD1 Antibody - Azide and BSA Free
Immunoprecipitation: AARSD1 Antibody [NBP3-00270] -
Immunoprecipitation: AARSD1 Antibody [NBP3-00270] - AARSD1 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate 2 uL NBP3-00270 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00270, at 1:100 dilution. Secondary antibody: IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 45 kDa Observed band size: 45 kDa
Western Blot: AARSD1 Antibody [NBP3-00270] -
Western Blot: AARSD1 Antibody [NBP3-00270] - NBP3-00270 at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:45 kDa Observed band size:45 kDa (We are unsure as to the identity of these extra bands.)Applications for AARSD1 Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AARSD1
Alternate Names
alanyl-tRNA editing protein Aarsd1, alanyl-tRNA synthetase domain containing 1, Alanyl-tRNA synthetase domain-containing protein 1, MGC2744
Gene Symbol
AARSD1
Additional AARSD1 Products
Product Documents for AARSD1 Antibody - Azide and BSA Free
Product Specific Notices for AARSD1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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