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PPARA/PPARD/PPARG Complete Transcription Factor Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1357

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KA1357

Key Product Details

Sample Type & Volume Required Per Well

Nuclear Extracts (10 uL/well. All samples should be assayed in duplicate at minimum.)

Product Specifications

Assay Type

Colorimetric

Kit Type

Assay Kit (Colorimetric)

Description

PPARA/PPARD/PPARG Complete Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting PPAR-alpha, delta, gamma transcription factor DNA binding activity in nuclear extracts and whole cell lysates.

Scientific Data Images

PPARA/PPARD/PPARG Complete Transcription Factor Assay Kit (Colorimetric) [KA1357] - Increasing amounts of positive control (total lysate) are assayed for PPARA, PPARD, and PPARG DNA-binding activity.

Kit Contents

  • 96-Well Cover Sheet
  • Polysorbate 20
  • Transcription Factor Antibody Binding Buffer (10X)
  • Transcription Factor Binding Assay Buffer (4X)
  • Transcription Factor Complete PPARalpha Positive Control
  • Transcription Factor Complete PPARalpha Primary Antibody
  • Transcription Factor Complete PPARdelta Positive Control
  • Transcription Factor Complete PPARdelta Primary Antibody
  • Transcription Factor Complete PPARgamma Positive Control
  • Transcription Factor Complete PPARgamma Primary Antibody
  • Transcription Factor Developing Solution
  • Transcription Factor Goat Anti-Rabbit HRP Conjugate
  • Transcription Factor PPAR 96-Well Strip Plate
  • Transcription Factor PPAR Specific Competitor dsDNA
  • Transcription Factor Reagent A
  • Transcription Factor Stop Solution
  • Wash Buffer Concentrate (400X)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PPARA/PPARD/PPARG Complete Transcription Factor

PPARA/PPARD/PPARG Complete Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting PPAR-alpha, delta, gamma transcription factor DNA binding activity in nuclear extracts and whole cell lysates.

Additional PPARA/PPARD/PPARG Complete Transcription Factor Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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