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ProteinSimple a Bio-Techne Brand

Wes System - Simple Westerns just got even simpler
A Reputation of Quality: The Bio-Techne Brand Story
Discontinued Product
ProteinSimple will continue service and support of the Wes platform through July 30th, 2026. If you are interested in Simple Western’s Wes Instrument, please view our Jess™ or Abby™ instruments. They feature all the same benefits as Wes with the addition of multiplexing and RePlex!

Wes™ has been discontinued as of July 30th, 2021. ProteinSimple will continue service and support of the Wes platform through July 30th, 2026. Additional Wes information and documents may be found below. If you are interested in the Simple Western™ Wes instrument, please view the comparison of Simple Western Jess™ and Abby™ instruments below. Simple Western assays with Jess and Abby have all the benefits of Wes with additional features like RePlex™ and Stellar™ High Sensitivity NIR/IR Detection.

Simple Western is a gel-free, blot-free, and hands-free protein characterization solution. Proteins are complex and most traditional protein characterization methods are inefficient or inadequate. Simple Western is your protein characterization problem-solver.

  • Is your sample too precious or too expensive?
  • Do you need better reproducibility?
  • Is time to answer too long?
  • Do you need a method that is quantitative?
  • Is your current method unable to handle the throughput you need?

Protein characterization should be easy and with Simple Western, it can be. Simple Western reinvents how Western blots are done, automating all steps from protein separation, immunoprobing, detection, and analysis of data. Simple Western finally offers researchers an analytical tool that delivers quantifiable and reproducible results in hours instead of days.

Which system is right for you?


Simple Western system Jess Abby Wes
Simple Western size assays
Simple Western charge assays      
Maximum samples per run 25 25 25
Run time for max samples (size) < 3 hours < 3 hours < 3 hours
Run time for max samples (charge) N/A N/A N/A
Sample cooling (size) N/A N/A N/A
Sample cooling (charge) N/A N/A N/A
Operating humidity range 20 – 60% relative, non-condensing
Operating temp range 18 – 24º C
Power 100 – 230 V AC, 50/60 Hz
Dimensions (H x W x D) 33 x 33 x 52 cm 33 x 33 x 52 cm 33 x 33 x 52 cm
Part number 004-650 004-680 004-600


Jess Instrument



Size assays on up to 25 samples

Chemiluminescence and Fluorescence

Includes RePlex

Includes Stellar High Sensitivity NIR / IR

Abby Instrument



Size assays on up to 25 samples


Includes RePlex

Citations for Wes

View all citations for the Simple Western platform.
Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Testimonials from your Peers for Simple Western

Showing  1 - 5 of 30 testimonials Showing All

"Simple Western™ allows us to run three-hour assays, which would usually take us two days with traditional Western blots."

Laura Martins, Ph.D., Early Development Scientist, Oxford Biomedica

"Jess allows us to immediately identify potential autophagy biomarkers as they are upregulated or downregulated in response to treatment. This allows us to use more advanced statistical techniques such as principal component analysis and hierarchal clustering to recognize key correlations in our research with ease"

John K. Blackwood PhD, Head of Biology, and Jakub Stefaniak PhD, Samsara Therapeutics

"The introduction of an Abby will enable us to answer important questions quicker, such as identifying the disease driving mechanism and molecules surrounding osteoarthritis. Moving away from the tedious manual western blotting process, which can take up to two days, and introducing automation will speed up time to data and provide increased sensitivity and accuracy."

Dr. Mario Rothbauer, Postdoc Researcher, Department of Orthopedics and Trauma Surgery, Medical University of Vienna

"It can be difficult to optimize new antibodies, particularly for brain markers where you have a lot of background noise and low levels of your target. The appropriate control and low variability between plates using Simple Western™ means we can complete our studies in weeks rather than months."

Dr. Michael Harte, Senior Lecturer in Drug Action, Neuroscience, University of Manchester

"One of the best advantages of using Wes is that it saves a lot of time, both in terms of hands-on time and in time to completion of the experiment. We were spending more than 4 hours of hands-on time per Western blot with traditional methods, and we can set up a run on the Wes in about 45 minutes. Our Wes runs are completed in less than 4 hours, as opposed to 4 days with traditional methods."

Noelle Anastasio, Ph.D., Assistant Professor, Department of Pharmacology and Toxicology, University of Texas Medical Branch

Showing  1 - 5 of 30 testimonials Showing All

More Information About Wes

Wes Applications

Cancer & Immuno-Oncology

Cancer & Immuno-Oncology

Transform your Cancer & Immuno-oncology research by utilizing next-generation western blotting tools to give you richer views into your samples.



Reduce your lab time with automated simple western systems while accelerating your COVID-19 research. Set up, leave and remotely review your data in just 3 hours!

Cell & Gene Therapy

Cell & Gene Therapy

Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.

Targeted Degradation

Targeted Degradation

Boost your screening studies using high throughput & hands-free Simple Western systems for quantitative analysis.

SARS-CoV-2 Serology

SARS-CoV-2 Serology

Quantitatively characterize serum or plasma human IgG antibodies reactive against 5 key viral antigens within one 3-hour run!



A validated workflow for protein analysis in extracellular vesicle samples from the leaders in exosome isolation and high sensitivity western blotting solutions.

Description Total Protein Specification Immunoassay Charge Specification
Sample Required 0.3-1.2 µg lysate 0.6-1.2 µg
Volume Required 3 µL/well 3 µL/well
Size or pI Range Molecular weight (MW) ladders range from 2-440 kDa Molecular weight (MW) ladders range from 2-440 kDa
Sizing CV <10% <10%
Intra-assay CV <15% <15%
Inter-assay CV <20%* <20%**
Resolution (± percent difference in MW) ± 15-20% for MW <20 kDa
±10 % for MW > 20 kDa
± 15-20% for MW <20 kDa
±10 % for MW > 20 kDa
Quantitation CV <20% <20%
Dynamic Range 2-3 logs 3 logs
Sensitivity ng Low pg
Capillary 5 cm, 100 µm, 400 nL
* inter-assay CV is with system control
** percent peak area

Product Documents for Wes

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