Proteome Profiler Human Angiogenesis Array Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # ARY007
Contains 4 membranes - each spotted in duplicate with 55 different angiogenesis antibodies
Key Product Details
Species
Human
Product Summary for Proteome Profiler Human Angiogenesis Array Kit
Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected angiogenesis-related proteins. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma and saliva.
Key Benefits
- Detects 55 human angiogenesis-related proteins simultaneously
- Requires no specialized equipment
- Compatible with LI-COR* and chemiluminescence detection
Principle of the Assay
The Proteome Profiler Human Angiogenesis Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of bound analyte. Analytes include soluble growth and differentiation factors, extracellular matrix components, proteases, membrane-bound receptors, and intracellular signaling molecules.
Why Use an Antibody Array to Detect Angiogenesis-Related Proteins?
Determining the expression of multiple proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.
Kit Contents
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Antibody Detection Cocktail
- Streptavidin-HRP
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
| Simultaneously detect the relative levels of these angiogenesis-related proteins in a single sample. | ||
|---|---|---|
| Activin A | FGF-7/KGF | PD-ECGF |
| ADAMTS-1 | GDNF | PDGF-AA |
| Angiogenin | GM-CSF | PDGF-AB/PDGF-BB |
| Angiopoietin-1 | HB-EGF | Persephin |
| Angiopoietin-2 | HGF | CXCL4/PF4 |
| Angiostatin/Plasminogen | IGFBP-1 | PlGF |
| Amphiregulin | IGFBP-2 | Prolactin |
| Artemin | IGFBP-3 | Serpin B5/Maspin |
| Tissue Factor/Factor III | IL-1 beta | Serpin E1/PAI-1 |
| CXCL16 | CXCL8/IL-8 | Serpin F1/PEDF |
| DPPIV/CD26 | LAP (TGF-beta 1) | TIMP-1 |
| EGF | Leptin | TIMP-4 |
| EG-VEGF | CCL2/MCP-1 | Thrombospondin-1 |
| Endoglin/CD105 | CCL3/MIP-1 alpha | Thrombospondin-2 |
| Endostatin/Collagen XVIII | MMP-8 | uPA |
| Endothelin-1 | MMP-9 | Vasohibin |
| FGF acidic | NRG1-beta 1 | VEGF |
| FGF basic | Pentraxin 3 | VEGF-C |
| FGF-4 | ||
Assays for analytes represented in the Human Angiogenesis Antibody Array Kit
| Quantikine® ELISA Kits | DuoSet® ELISA Development Systems | Quantikine® HS (High Sensitivity) ELISA Kits | QuantiGlo® Chemiluminescent ELISA Kits | |
|---|---|---|---|---|
| Activin A | DAC00B | DY338 | ||
| ADAMTS-1 | DY2197 | |||
| Angiogenin | DAN00 | DY265 | ||
| Angiopoietin-1 | DANG10 | DY923 | ||
| Angiopoietin-2 | DANG20 | DY623 | ||
| Angiostatin/Plasminogen | ||||
| Amphiregulin | DY262 | |||
| Artemin | DY2589 | |||
| Tissue Factor/Factor III | DCF300 | DY2339 | ||
| CXCL16 | DCX160 | DY1164 | ||
| DPPIV/CD26 | DY1180 | |||
| EGF | DEG00 | DY236 | ||
| EG-VEGF | DY1209 | |||
| Endoglin | DNDG00 | DY1097 | ||
| Endostatin/Collagen XVII | DNST0 | DY1098 | ||
| Endothelin-1 | DET100 | QET00B | ||
| FGF acidic | DFA00B | DY232 | ||
| FGF basic | DFB50 | DY233 | HSFB00D | |
| FGF-4 | DY235 | |||
| FGF-7/KGF | DKG00 | DY251 | ||
| GDNF | DY212 | |||
| GM-CSF | DGM00 | DY215 | HSGM0 | |
| HB-EGF | DY259 | |||
| HGF | DHG00 | DY294 | ||
| IGFBP-1 | DY871 | |||
| IGFBP-2 | DGB200 | DY674 | ||
| IGFBP-3 | DGB300 | DY675 | ||
| IL-1 beta | DLB50 | DY201 | HSLB00D | QLB00B |
| CXCL8/IL-8 | D8000C | DY208 | HS800 | Q8000B |
| TGF-beta1 (LAP) | DB100B | DY240 | ||
| Leptin | DLP00 | DY398 | ||
| CCL2/MCP-1 | DCP00 | DY279 | ||
| CCL3/MIP-1 alpha | DMA00 | DY270 | ||
| MMP-8 | DMP800 | DY908 | ||
| MMP-9 | DMP900 | DY911 | ||
| NRG1-beta1 | DY377 | |||
| Pentraxin 3 | DPTX30 | DY1826 | ||
| PD-ECGF | ||||
| PDGF-AA | DAA00B | DY221 | ||
| PDGF-AB/PDGF-BB | DHD00C, DBB00 | DY222, DY220 | ||
| Persephin | ||||
| CXCL4/PF4 | DY795 | |||
| PlGF | DPG00 | DY264 | ||
| Prolactin | DPRL00 | DY682 | ||
| Serpin B5/Maspin | ||||
| Serpin E1/PAI-1 | DSE100 | DY1786 | ||
| Serpin F1/PEDF | ||||
| TIMP-1 | DTM100 | DY970 | ||
| TIMP-4 | DTM400 | DY974 | ||
| Thrombospondin-1 | DTSP10 | DY3074 | ||
| Thrombospondin-2 | DTSP20 | DY1635 | ||
| uPA | DUPA00 | DY1310 | ||
| Vasohibin | ||||
| VEGF | DVE00 | DY293B | QVE00B | |
| VEGF-C | DVEC00 | DY752B |
Scientific Data Images for Proteome Profiler Human Angiogenesis Array Kit
Profiling Angiogenesis-Related Proteins in Human Cancer Tissues.
A) The Proteome Profiler Human Angiogenesis Antibody Array (Catalog # ARY007) was used to profile angiogenesis-related proteins in tissues from human prostate, ovarian, and breast cancers. B) Histogram profiles for select analytes were generated by quantifying the mean spot pixel densities from the array membrane using image software.
The Human Angiogenesis Array detects multiple analytes in cell culture supernates.
A. THP-1 human acute monocytic leukemia cell line treated with 1 µg/mL Recombinant Human IFN-gamma (Catalog # 285-IF) for 8 hours and 1 µg/mL lipopolysaccharide (LPS) for 16 hours. B. HUVEC human umbilical vein endothelial cells treated with 10 mg/ml of LPS for 24 hours. 500 µL of supernate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.
The Human Angiogenesis Array detects multiple analytes in tissue lysate, serum, and plasma.
Representative membranes and selective quantification are shown for human ovarian cancer tissue lysate (A., 200 µg), serum (B., 100 µL), and EDTA plasma (C., 100 µL). Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.Formulation, Preparation, and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Angiogenesis Assay Kits
Angiogenesis is the tightly regulated process by which new blood vessels are formed from the existing vasculature. This process is physiologically important for development and wound healing, and is also a common driver in multiple diseases including rheumatoid arthritis, atherosclerosis, macular degeneration, and cancer. Angiogenesis occurs in response to a variety of molecular cues. Generally, the angiogenic process includes endothelial cell proliferation, chemotactic endothelial cell migration through the extracellular matrix barrier, and the formation of capillary tubes. Physiological and pathological angiogenesis utilize many of the same cellular processes and molecular signaling networks, however the structures that form during pathological angiogenesis are often functionally abnormal.
Additional Angiogenesis Assay Kits Products
Product Documents for Proteome Profiler Human Angiogenesis Array Kit
Product Specific Notices for Proteome Profiler Human Angiogenesis Array Kit
For research use only
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