Human KGF/FGF-7 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DKG00
Key Product Details
Assay Length
5.25 hours or 5.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL)
Sensitivity
15 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human KGF/FGF-7 Quantikine ELISA Kit
The Quantikine Human KGF Immunoassay is a 5.25 or 5.5 hour solid phase ELISA designed to measure KGF in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human KGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant KGF accurately. Results obtained measuring natural human KGF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human KGF.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human KGF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 82.4 | 248 | 1031 | 86.5 | 266 | 1108 |
| Standard Deviation | 3.2 | 8.8 | 55.2 | 4.8 | 11.4 | 62.0 |
| CV% | 3.9 | 3.5 | 5.4 | 5.5 | 4.3 | 5.6 |
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 97.9 | 298 | 1229 | 95.5 | 294 | 1221 |
| Standard Deviation | 3.3 | 8.8 | 42.8 | 7.4 | 15.6 | 63.5 |
| CV% | 3.4 | 3.0 | 3.5 | 7.7 | 5.3 | 5.2 |
Recovery for Human KGF/FGF-7 Quantikine ELISA Kit
The recovery of KGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 102 | 95-105 |
| Citrate Plasma (n=5) | 91 | 86-98 |
| EDTA Plasma (n=5) | 96 | 85-104 |
| Heparin Plasma (n=5) | 90 | 85-99 |
| Serum (n=5) | 93 | 85-100 |
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of KGF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human KGF/FGF-7 Quantikine ELISA Kit
Human KGF/FGF-7 ELISA Cell Culture Supernate Standard Curve
Human KGF/FGF-7 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: KGF/FGF-7
KGF (keratinocyte growth factor), also known as FGF-7, is induced in mesenchymal cells by inflammation or tissue damage. It functions as a paracrine factor during wound healing by inducing the proliferation of nearby epithelial cells. KGF plays a role in kidney morphogenesis, hair follicle development, and keratinocyte differentiation. It signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (IIIb)/KGF R.
Long Name
Keratinocyte Growth Factor
Alternate Names
FGF-7, FGF7, HBGF-7, HBGF7
Gene Symbol
FGF7
Additional KGF/FGF-7 Products
Product Documents for Human KGF/FGF-7 Quantikine ELISA Kit
Product Specific Notices for Human KGF/FGF-7 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.