Human PDGF-AB Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DHD00C
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (10 µL), Platelet-poor EDTA Plasma (100 µL), Platelet-poor Heparin Plasma (100 µL)
Sensitivity
3.83 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
Product Summary for Human PDGF-AB Quantikine ELISA Kit
The Quantikine PDGF-AB Immunoassay is a 4.5 hour solid phase ELISA designed to measure human PDGF-AB in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant human PDGF-AB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-AB showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-AB.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human PDGF-AB
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 92.7 | 312 | 656 | 102 | 343 | 701 |
| Standard Deviation | 8.28 | 9.73 | 15.2 | 10.9 | 29.9 | 52.3 |
| CV% | 8.9 | 3.1 | 2.3 | 10.7 | 8.7 | 7.5 |
Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 107 | 350 | 726 | 119 | 362 | 723 |
| Standard Deviation | 6.95 | 10.9 | 63.1 | 10.7 | 20.9 | 54.5 |
| CV% | 6.5 | 3.1 | 8.7 | 9.0 | 5.8 | 7.5 |
Recovery for Human PDGF-AB Quantikine ELISA Kit
The recovery of PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 105 | 93-119 |
| Platelet-poor EDTA Plasma (n=4) | 104 | 78-117 |
| Platelet-poor Heparin Plasma (n=4) | 105 | 86-121 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PDGF-AB were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human PDGF-AB Quantikine ELISA Kit
Human PDGF-AB ELISA Cell Culture Supernate Standard Curve
Human PDGF-AB ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PDGF-AB
PDGF-A and PDGF-B are platelet-derived growth factors that can form disulfide-linked homodimers or heterodimers with each other. These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of PDGF R alpha and R beta. PDGF-AB binds to alpha receptor homodimers as well as the heterodimeric receptor. PDGF-AA and -AB promote cell proliferation, cell survival, cytoskeletal rearrangement, cell migration and extracellular matrix production. They are important for the development of oligodendrocytes, testicular Leydig cells, alveolar smooth muscle cells, hair follicles, and intestinal villus.
Long Name
Platelet-derived Growth Factor AB
Alternate Names
PDGFAB
Additional PDGF-AB Products
Product Documents for Human PDGF-AB Quantikine ELISA Kit
Product Specific Notices for Human PDGF-AB Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.