Human Angiogenin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DAN00
Key Product Details
Assay Length
2.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL)
Sensitivity
6 pg/mL
Assay Range
78.1-5000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human Angiogenin Quantikine ELISA Kit
The Quantikine Human Angiogenin Immunoassay is a 2.5 hour solid phase ELISA designed to measure Angiogenin in cell culture supernates, serum, and plasma. It contains recombinant human Angiogenin and antibodies raised against the recombinant factor. It has been shown to accurately quantitate both natural and recombinant human Angiogenin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Angiogenin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 356 | 717 | 1739 | 664 | 1911 | 3671 |
| Standard Deviation | 10 | 24 | 50 | 47 | 166 | 309 |
| CV% | 2.8 | 3.3 | 2.9 | 7.1 | 8.7 | 8.4 |
Recovery for Human Angiogenin Quantikine ELISA Kit
The recovery of Angiogenin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 107 | 97-117 |
| Citrate Plasma (n=10) | 102 | 84-126 |
| EDTA Plasma (n=10) | 101 | 79-121 |
| Heparin Plasma (n=10) | 102 | 80-137 |
| Serum (n=10) | 103 | 84-126 |
Linearity
The following samples were diluted with Calibrator Diluent and assayed to assess linearity of the assay.
Scientific Data Images for Human Angiogenin Quantikine ELISA Kit
Human Angiogenin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Angiogenin
Angiogenin (ANG) is a secreted protein that belongs to the pancreatic ribonuclease family. It was initially purified based on its ability to initiate vascularization in the chicken embryo chorioallantoic membrane from serum-free media conditioned by growth of a human adenocarcinoma cell line HT-29. A number of other tumors, as well as normal cell lines, also secrete Angiogenin. Angiogenin is present in normal human plasma.
Additional Angiogenin Products
Product Documents for Human Angiogenin Quantikine ELISA Kit
Product Specific Notices for Human Angiogenin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.