Human Angiopoietin-2 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DANG20
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (50 µL)
Sensitivity
21.3 pg/mL
Assay Range
46.9-3000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Product Summary for Human Angiopoietin-2 Quantikine ELISA Kit
The Quantikine Human Angiopoietin-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Angiopoietin-2 in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human Angiopoietin-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Angiopoietin-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Angiopoietin-2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Angiopoietin-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human Angiopoietin-2 in this assay. No medical histories were available for the donors used
in this study.
Saliva - Nine samples were evaluated for the presence of human Angiopoietin-2 in this assay and ranged from 247-822 pg/mL.
Note: Significant levels of Angiopoietin-2 are found in fetal bovine, bovine, porcine, equine, and rabbit serum. The background level of Angiopoietin-2 in control medium should be determined and subtracted from samples of conditioned medium.
| Sample Type | Mean (pg/mL) | Range (pg/mL) | Standard Deviation (pg/mL) |
| Serum (n=60) | 2494 | 1065-8907 | 1341 |
| EDTA plasma (n=35) | 1964 | 1071-4389 | 808 |
| Heparin plasma (n=35) | 2049 | 1009-4973 | 913 |
Saliva - Nine samples were evaluated for the presence of human Angiopoietin-2 in this assay and ranged from 247-822 pg/mL.
Cell Culture Supernates - Two cultures of HUVEC human umbilical vein endothelial cells were
grown to confluency in EGM supplemented with 2% fetal bovine serum, 2 mM L-glutamine,
100 U/mL penicillin, 100 μg/mL streptomycin sulfate and bovine brain extract. Aliquots of the
cell culture supernates were removed and assayed for levels of human Angiopoietin-2.
| Sample | Values (pg/mL) |
| HUVEC Culture 1 | 2326 |
| HUVEC Culture 2 | 7611 |
Note: Significant levels of Angiopoietin-2 are found in fetal bovine, bovine, porcine, equine, and rabbit serum. The background level of Angiopoietin-2 in control medium should be determined and subtracted from samples of conditioned medium.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 237 | 703 | 1301 | 276 | 805 | 1494 |
| Standard Deviation | 16.4 | 45.6 | 54.4 | 28.8 | 73.0 | 111 |
| CV% | 6.9 | 6.5 | 4.2 | 10.4 | 9.1 | 7.4 |
Recovery for Human Angiopoietin-2 Quantikine ELISA Kit
The recovery of Angiopoietin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 98-113 |
| EDTA Plasma (n=4) | 95 | 85-104 |
| Heparin Plasma (n=4) | 94 | 87-104 |
| Serum (n=4) | 100 | 90-107 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Angiopoietin-2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Angiopoietin-2 Quantikine ELISA Kit
Human Angiopoietin-2 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Angiopoietin-2
Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that regulates vascular remodeling by promoting endothelial cell survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells. Ang-2 is required for postnatal vascular remodeling, and it cooperates with Ang-1 during lymphatic vessel development. It mediates the upregulation of ICAM-1 and VCAM-1 on EC, which facilitates the adhesion of leukocytes during inflammation. Its direct interaction with Integrins promotes tumor cell invasion. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It associates into dimers, trimers, and larger multimers and binds the receptor Tie-2, with its function as an agonist or antagonist depending on its state of multimerization.
Alternate Names
ANGPT2
Entrez Gene IDs
Gene Symbol
ANGPT2
Additional Angiopoietin-2 Products
Product Documents for Human Angiopoietin-2 Quantikine ELISA Kit
Product Specific Notices for Human Angiopoietin-2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.