Human Coagulation Factor III/Tissue Factor Quantikine ELISA
R&D Systems, part of Bio-Techne | Catalog # DCF300
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Citrate Plasma (50 µL), Urine (50 µL)
Sensitivity
2.05 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Product Summary for Human Coagulation Factor III/Tissue Factor Quantikine ELISA
The Quantikine Human Coagulation Factor III/Tissue Factor Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Coagulation Factor III in cell culture supernates, cell lysates, plasma, and urine. It contains NS0-expressed recombinant human Coagulation Factor III and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Coagulation Factor III showed linear curves that were p arallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Coagulation Factor III.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Coagulation Factor III
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 33.4 | 65.4 | 148 | 71 | 144 | 301 |
| Standard Deviation | 0.78 | 1.81 | 5.0 | 4.5 | 7.8 | 17.1 |
| CV% | 2.3 | 2.8 | 3.4 | 6.3 | 5.4 | 5.7 |
Recovery for Human Coagulation Factor III/Tissue Factor Quantikine ELISA
The recovery of Coagulation Factor III spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 104 | 95-110 |
| Citrate Plasma (n=4) | 92 | 86-102 |
| EDTA Plasma (n=4) | 91 | 86-97 |
| Heparin Plasma (n=4) | 95 | 88-109 |
| Urine (n=4) | 102 | 96-112 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Coagulation Factor III were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Coagulation Factor III/Tissue Factor Quantikine ELISA
Human Coagulation Factor III/Tissue Factor ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Coagulation Factor III/Tissue Factor
Coagulation Factor III, also known as Tissue Factor (TF), Thromboplastin, and CD142, is the primary initiator of the extrinsic coagulation pathway. It is a transmembrane protein that is consitutively expressed in subendothelial cells throughout the vasculature and is inducible on endothelial cells and monocytes. Damage to the vessel wall exposes Coagulation Factor III to circulating Factor VII, triggering the proteolytic cascade that results in fibrin clot formation. Coagulation Factor III plays an important role in thrombotic diseases, disseminated intravascular coagulation, and atherosclerosis. It also plays a role in non-hemostatic inflammation, angiogenesis, and tumor progression.
Alternate Names
CD142, F3, Thromboplastin, Tissue Factor
Gene Symbol
F3
Additional Coagulation Factor III/Tissue Factor Products
Product Documents for Human Coagulation Factor III/Tissue Factor Quantikine ELISA
Product Specific Notices for Human Coagulation Factor III/Tissue Factor Quantikine ELISA
For research use only
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.