Human/Mouse/Rat/Hamster ACE‑2 Antibody Best Seller
Catalog # AF933 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Human/Mouse/Rat/Hamster ACE‑2 Antibody
Immunogen
Gln18-Ser740
Accession # Q9BYF1
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse/Rat/Hamster ACE‑2 Antibody
Detection of Human, Mouse, and Rat ACE‑2 by Western Blot.
Western blot shows lysates of human kidney tissue, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ACE-2 at approximately 100 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Detection of ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF933) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). Quadrant markers were set based on Goat IgG control antibody (AB-108-C, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.ACE‑2 in Human Kidney.
ACE-2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse/Rat/Hamster ACE‑2 Antibody
Blockade of Receptor-ligand Interaction
Flow Cytometry
Sample: HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP
Immunohistochemistry
Sample: Immersion fixed frozen sections of rat lung, immersion fixed paraffin-embedded sections of human kidney, hamster lung, rat lung, and rat kidney
Immunoprecipitation
Sample: Conditioned cell culture medium spiked with Recombinant Human ACE‑2 (Catalog # 933-ZN), see our available Western blot detection antibodies
Simple Western
Sample: Human kidney tissue
Western Blot
Sample: Human kidney tissue, Mouse kidney tissue, and Rat kidney tissue
Reviewed Applications
Read 4 reviews rated 5 using AF933 in the following applications:
- Block/Neutralize (1 Review)
- Immunocytochemistry/Immunofluorescence (1 Review)
- Immunofluorescence (1 Review)
- Western Blot (1 Review)
Published Applications
Read 117 publications using AF933 in the following applications:
- Co-Immunoprecipitation (2 Publications)
- Control (1 Publication)
- ELISA Detection (1 Publication)
- FLOW (1 Publication)
- Flow Cytometry (32 Publications)
- ICC (26 Publications)
- IHC (28 Publications)
- IHC-Fr (1 Publication)
- IHC-P (2 Publications)
- Neutralization (15 Publications)
- Western Blot (37 Publications)
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ACE-2
ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).
ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (4, 5). This Human anti-ACE2 antibody (catalog # AF933) was used to block the variant SARS-CoV-2 and ACE2 interaction to elucidate viral transmission and potential therapeutic strategies. (5)
References
- Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
- Crackower, M.A. et al. (2002) Nature 417:822.
- Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
- Li, W. et al. (2003) Nature 426:450.
- Hoffmann, M. et al. (2020) Cell. DOI: 10.1016/j.cell.2020.02.052.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Product Documents for Human/Mouse/Rat/Hamster ACE‑2 Antibody
Product Specific Notices for Human/Mouse/Rat/Hamster ACE‑2 Antibody
For research use only
Citations for Human/Mouse/Rat/Hamster ACE‑2 Antibody (117)
Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat/Hamster ACE‑2 Antibody include:
-
Species: Human
Sample Types: Whole Cells
Applications: ICCMinami et al. (2023-07-18), Effective SARS-CoV-2 replication of monolayers of intestinal epithelial cells differentiated from human induced pluripotent stem cells Scientific reports
PMID: 37463955 -
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHCLiu et al. (2023-06-20), An inducible hACE2 transgenic mouse model recapitulates SARS-CoV-2 infection and pathogenesis in vivo Proceedings of the National Academy of Sciences of the United States of America
PMID: 37307455 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryKhan et al. (2023-05-30), Generation of a SARS-CoV-2 Reverse Genetics System and Novel Human Lung Cell Lines That Exhibit High Virus-Induced Cytopathology Viruses
PMID: 37376581 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationBarreto et al. (2023-05-23), COVID-19-related hyperglycemia is associated with infection of hepatocytes and stimulation of gluconeogenesis Proceedings of the National Academy of Sciences of the United States of America
PMID: 37186819 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryKummarapurugu et al. (2023-05-13), Neutrophil Elastase decreases SARS-CoV-2 Spike protein binding to human bronchial epithelia by clipping ACE-2 ectodomian from the epithelial surface The Journal of biological chemistry
PMID: 37187291 -
Species: Human
Sample Types: Whole Cells
Applications: FLOWM Ulinici et al. (2023-03-13), Characterisation of the Antibody Response in Sinopharm (BBIBP-CorV) Recipients and COVID-19 Convalescent Sera from the Republic of Moldova Vaccines, 2023-011(3).
PMID: 36992221 -
Species: Mouse
Sample Types: Whole Tissue,Tissue Homogenates
Applications: Western Blot,IHCAT Tang et al. (2023-02-06), Cell-autonomous requirement for ACE2 across organs in lethal mouse SARS-CoV-2 infection PloS Biology, 2023-021(2):e3001989.
PMID: 36745682 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotN Tailor et al. (2022-12-28), Generation and Characterization of a SARS-CoV-2-Susceptible Mouse Model Using Adeno-Associated Virus (AAV6.2FF)-Mediated Respiratory Delivery of the Human ACE2 Gene Viruses, 2022-115(1).
PMID: 36680125 -
Species: Human
Sample Types: Whole Cells,Cell Lysates,Whole Tissue
Applications: Co-Immunoprecipitation,IHC,Flow Cytometry,Western BlotA Miluzio et al. (2022-12-28), Mapping of functional SARS-CoV-2 receptors in human lungs establishes differences in variant binding and SLC1A5 as a viral entry modulator of hACE2 EBioMedicine, 2022-187(0):104390.
PMID: 36584595 -
Species: Human
Sample Types: Whole Cells
Applications: ICCX Guo et al. (2022-12-23), Control of SARS-CoV-2 infection by MT1-MMP-mediated shedding of ACE2 Nature Communications, 2022-113(1):7907.
PMID: 36564389 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotY Kato et al. (2022-12-21), TRPC3-Nox2 Protein Complex Formation Increases the Risk of SARS-CoV-2 Spike Protein-Induced Cardiomyocyte Dysfunction through ACE2 Upregulation International Journal of Molecular Sciences, 2022-124(1).
PMID: 36613540 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotSB Biering et al. (2022-12-09), SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-beta signaling Nature Communications, 2022-113(1):7630.
PMID: 36494335 -
Species: Mink
Sample Types: Whole Tissue
Applications: IHCDR Adney et al. (2022-11-22), Severe acute respiratory disease in American mink experimentally infected with SARS-CoV-2 JCI Insight, 2022-17(22).
PMID: 36509288 -
Species: Human,Bat
Sample Types: Whole Cells
Applications: NeutralizationH Guo et al. (2022-11-21), ACE2-Independent Bat Sarbecovirus Entry and Replication in Human and Bat Cells MBio, 2022-10(0):e0256622.
PMID: 36409074 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryJ Song et al. (2022-10-13), LRRC15 inhibits SARS-CoV-2 cellular entry in trans PloS Biology, 2022-120(10):e3001805.
PMID: 36228039 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotK Shilagardi et al. (2022-10-05), The Integral Membrane Protein ZMPSTE24 Protects Cells from SARS-CoV-2 Spike-Mediated Pseudovirus Infection and Syncytia Formation MBio, 2022-10(0):e0254322.
PMID: 36197088 -
Species: African Green Monkey
Sample Types: Whole Cells
Applications: ICCTD Saccon et al. (2022-09-29), SARS-CoV-2 infects adipose tissue in a fat depot- and viral lineage-dependent manner Nature Communications, 2022-013(1):5722.
PMID: 36175400 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryN Brasu et al. (2022-09-22), Memory CD8+ T cell diversity and B cell responses correlate with protection against SARS-CoV-2 following mRNA vaccination Nature Immunology, 2022-00(0).
PMID: 36138186 -
JV Harte et al. (2022-09-21), Metalloprotease-Dependent S2'-Activation Promotes Cell-Cell Fusion and Syncytiation of SARS-CoV-2 Viruses, 2022-014(10).
PMID: 36298651 -
Species: Human
Sample Types: Whole Cells
Applications: Control,ICCM Montano et al. (2022-09-14), SARS-CoV-2 can infect human embryos Scientific Reports, 2022-012(1):15451.
PMID: 36104397 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotY Yao et al. (2022-09-09), Surface translocation of ACE2 and TMPRSS2 upon TLR4/7/8 activation is required for SARS-CoV-2 infection in circulating monocytes Cell Discovery, 2022-08(1):89.
PMID: 36085197 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotJ Hou et al. (2022-08-15), Integrated multi-omics analyses identify key anti-viral host factors and pathways controlling SARS-CoV-2 infection Research square, 2022-00(0).
PMID: 36032971 -
Species: Human,Mouse
Sample Types: Cell Lysates,Whole Tissue
Applications: Co-Immunoprecipitation,Western Blot,IHCP Su et al. (2022-08-09), Development of a novel peptide to prevent entry of SARS-CoV-2 into lung and olfactory bulb cells of hACE2 expressing mice Molecular Brain, 2022-015(1):71.
PMID: 35945596 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationB Meng et al. (2022-08-03), SARS-CoV-2 spike N-terminal domain modulates TMPRSS2-dependent viral entry and fusogenicity Cell Reports, 2022-040(7):111220.
PMID: 35963244 -
Species: Human
Sample Types: Whole Cells,Cell Lysates
Applications: ICC,Western BlotY Zhu et al. (2022-08-01), Ancestral SARS-CoV-2, but not Omicron, replicates less efficiently in primary pediatric nasal epithelial cells PloS Biology, 2022-020(8):e3001728.
PMID: 35913989 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCH Stölting et al. (2022-07-15), Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1 Mucosal Immunology, 2022-00(0).
PMID: 35840680 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryCW Chang et al. (2022-06-23), A Newly Engineered A549 Cell Line Expressing ACE2 and TMPRSS2 Is Highly Permissive to SARS-CoV-2, Including the Delta and Omicron Variants Viruses, 2022-014(7).
PMID: 35891350 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCL Casciola-R et al. (2022-05-09), IgM anti-ACE2 autoantibodies in severe COVID-19 activate complement and perturb vascular endothelial function JCI Insight, 2022-07(9).
PMID: 35349483 -
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,NeutralizationC Junqueira et al. (2022-04-06), FcgammaR-mediated SARS-CoV-2 infection of monocytes activates inflammation Nature, 2022-00(0).
PMID: 35385861 -
Species: Human
Sample Types: Organoid
Applications: IHCP Spitalieri et al. (2022-04-05), Two Different Therapeutic Approaches for SARS-CoV-2 in hiPSCs-Derived Lung Organoids Cells, 2022-011(7).
PMID: 35406799 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryM Ramal-Sanc et al. (2022-03-28), ACE2 Receptor and Its Isoform Short-ACE2 Are Expressed on Human Spermatozoa International Journal of Molecular Sciences, 2022-023(7).
PMID: 35409054 -
Species: Human
Sample Types: Organoid
Applications: NeutralizationY Menuchin-L et al. (2022-03-24), SARS-CoV-2 infects and replicates in photoreceptor and retinal ganglion cells of human retinal organoids Stem Cell Reports, 2022-00(0).
PMID: 35334213 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryX Wang et al. (2022-02-17), Diltiazem inhibits SARS-CoV-2 cell attachment and internalization and decreases the viral infection in mouse lung PloS Pathogens, 2022-018(2):e1010343.
PMID: 35176124 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationS Krasemann et al. (2022-01-20), The blood-brain barrier is dysregulated in COVID-19 and serves as a CNS entry route for SARS-CoV-2 Stem Cell Reports, 2022-00(0).
PMID: 35063125 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotL El-Shennaw et al. (2022-01-20), Circulating ACE2-expressing extracellular vesicles block broad strains of SARS-CoV-2 Nature Communications, 2022-013(1):405.
PMID: 35058437 -
Species: Human
Sample Types: Whole Cells,Whole Tissue
Applications: IHC,ICCJ Grau-Expós et al. (2022-01-13), Evaluation of SARS-CoV-2 entry, inflammation and new therapeutics in human lung tissue cells PloS Pathogens, 2022-018(1):e1010171.
PMID: 35025963 -
Species: Human
Sample Types: Organoids
Applications: IHCL Helms et al. (2021-12-22), Cross-validation of SARS-CoV-2 responses in kidney organoids and clinical populations JCI Insight, 2021-10(0).
PMID: 34767537 -
Species: Human,Primate - C. aethiops
Sample Types: Whole Cells
Applications: Neutralization,Flow CytometryJ Wang et al. (2021-12-14), SARS-CoV-2 uses metabotropic glutamate receptor subtype 2 as an internalization factor to infect cells Cell Discovery, 2021-17(1):119.
PMID: 34903715 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotSB Biering et al. (2021-12-13), SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-beta signaling bioRxiv : the preprint server for biology, 2021-10(0).
PMID: 34931188 -
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA DetectionJ Shi et al. (2021-11-24), Receptor-Binding Domain Proteins of SARS-CoV-2 Variants Elicited Robust Antibody Responses Cross-Reacting with Wild-Type and Mutant Viruses in Mice Vaccines, 2021-19(12).
PMID: 34960131 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryL Nguyen et al. (2021-11-09), Sialic acid-containing glycolipids mediate binding and viral entry of SARS-CoV-2 Nature Chemical Biology, 2021-10(0).
PMID: 34754101 -
Species: Transgenic Mouse
Sample Types: Cell Lysates
Applications: Western BlotMW Ku et al. (2021-10-25), Brain Cross-Protection against SARS-CoV-2 Variants by a Lentiviral Vaccine in New Transgenic Mice Embo Molecular Medicine, 2021-10(0):e14459.
PMID: 34647691 -
Species: Human
Sample Types: Organoids
Applications: IHCX Duan et al. (2021-10-15), An airway organoid-based screen identifies a role for the HIF1alpha-glycolysis axis in SARS-CoV-2 infection Cell Reports, 2021-137(6):109920.
PMID: 34731648 -
Species: Human
Sample Types: Whole Tissue,Whole Cells
Applications: ICC,IHCS Singh et al. (2021-09-25), SARS-CoV-2 and its variants of concern infect human conjunctival epithelial cells and induce differential antiviral innate immune response The ocular surface, 2021-00(0).
PMID: 34583089 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotR Tian et al. (2021-09-20), BRD2 inhibition blocks SARS-CoV-2 infection in vitro by reducing transcription of the host cell receptor ACE2 bioRxiv : the preprint server for biology, 2021-00(0).
PMID: 33501440 -
Species: Human
Sample Types: Whole Cells,Cell Lysates
Applications: Western Blot,Flow CytometrySP Shukla et al. (2021-08-20), "Molecular Masks" for ACE2 to Effectively and Safely Block SARS-CoV-2 Virus Entry International Journal of Molecular Sciences, 2021-022(16).
PMID: 34445669 -
Species: Human
Sample Types: Whole Cells,Cell Lysates
Applications: Western Blot,Flow CytometryEJ Sherman et al. (2021-08-05), ACE2 protein expression within isogenic cell lines is heterogeneous and associated with distinct transcriptomes Scientific Reports, 2021-011(1):15900.
PMID: 34354120 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryM Alenquer et al. (2021-08-05), Signatures in SARS-CoV-2 spike protein conferring escape to neutralizing antibodies PloS Pathogens, 2021-017(8):e1009772.
PMID: 34352039 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCY Kondo et al. (2021-07-22), L-SIGN is a receptor on liver sinusoidal endothelial cells for SARS-CoV-2 virus JCI Insight, 2021-06(14).
PMID: 34291736 -
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: ICC,Western BlotCLC Almeida-da et al. (2021-07-18), Cigarette Smoke Stimulates SARS-CoV-2 Internalization by Activating AhR and Increasing ACE2 Expression in Human Gingival Epithelial Cells International Journal of Molecular Sciences, 2021-022(14).
PMID: 34299289 -
Species: Human
Sample Types: Organoid
Applications: IHCL Wang et al. (2021-07-09), A human three-dimensional neural-perivascular 'assembloid' promotes astrocytic development and enables modeling of SARS-CoV-2 neuropathology Nature Medicine, 2021-00(0).
PMID: 34244682 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotM Zhao et al. (2021-06-24), Rapid, reliable, and reproducible cell fusion assay to quantify SARS-Cov-2 spike interaction with hACE2 PloS Pathogens, 2021-017(6):e1009683.
PMID: 34166473 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryX Wang et al. (2021-06-23), Deletion of ER-retention motif on SARS-CoV-2 spike protein reduces cell hybrid during cell-cell fusion Cell & bioscience, 2021-011(1):114.
PMID: 34162440 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotM Puray-Chav et al. (2021-06-23), Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell Cell Reports, 2021-00(0):109364.
PMID: 34214467 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCM Baratchian et al. (2021-05-27), Androgen regulation of pulmonary AR, TMPRSS2 and ACE2 with implications for sex-discordant COVID-19 outcomes Scientific Reports, 2021-011(1):11130.
PMID: 34045511 -
Species: Human
Sample Types: Whole Tissue
Applications: ICCT Ebisudani et al. (2021-05-19), Direct derivation of human alveolospheres for SARS-CoV-2 infection modeling and drug screening Cell Reports, 2021-00(0):109218.
PMID: 34038715 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCAZ Eriksen et al. (2021-05-17), SARS-CoV-2 infects human adult donor eyes and hESC-derived ocular epithelium Cell Stem Cell, 2021-00(0).
PMID: 34022129 -
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,ICCCH Chen et al. (2021-04-09), Deletion of ER-retention Motif on SARS-CoV-2 Spike Protein Reduces Cell Hybrid During Cell-cell Fusion Research square, 2021-00(0).
PMID: 33851149 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotM Dittmar et al. (2021-03-23), Drug repurposing screens reveal cell-type-specific entry pathways and FDA-approved drugs active against SARS-Cov-2 Cell Reports, 2021-035(1):108959.
PMID: 33811811 -
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: Western Blot,Flow CytometrySJ Dicken et al. (2021-03-22), Characterisation of B.1.1.7 and Pangolin coronavirus spike provides insights on the evolutionary trajectory of SARS-CoV-2 bioRxiv : the preprint server for biology, 2021-00(0).
PMID: 33791702 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryY Zhang et al. (2021-03-15), SARS-CoV-2 hijacks folate and one-carbon metabolism for viral replication Nature Communications, 2021-012(1):1676.
PMID: 33723254 -
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: Western Blot,Flow CytometryJ Baggen et al. (2021-03-08), Genome-wide CRISPR screening identifies TMEM106B as a proviral host factor for SARS-CoV-2 Nature Genetics, 2021-00(0).
PMID: 33686287 -
Species: Human,Primate
Sample Types: Whole Cells
Applications: Flow Cytometry,ICCK Uemura et al. (2021-03-08), MRC5 cells engineered to express ACE2 serve as a model system for the discovery of antivirals targeting SARS-CoV-2 Scientific Reports, 2021-011(1):5376.
PMID: 33686154 -
Species: Rat
Sample Types: Whole Tissue,Tissue Homogenates
Applications: IHCEE Khoury et al. (2021-03-04), Pulmonary, cardiac and renal distribution of ACE2, furin, TMPRSS2 and ADAM17 in rats with heart failure: Potential implication for COVID-19 disease Journal of Cellular and Molecular Medicine, 2021-00(0).
PMID: 33660945 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCS Sugimoto et al. (2021-02-24), An organoid-based organ-repurposing approach to treat short bowel syndrome Nature, 2021-00(0).
PMID: 33627870 -
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,ICCH Huerga Enc et al. (2021-02-05), Human Erythroid Progenitors Are Directly Infected by SARS-CoV-2: Implications for Emerging Erythropoiesis in Severe COVID-19 Patients Stem Cell Reports, 2021-016(3):428-436.
PMID: 33581053 -
Species: Human
Sample Types: Whole Cells
Applications: ICCX Yin et al. (2021-01-12), MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells Cell Reports, 2021-034(2):108628.
PMID: 33440148 -
Species: Human
Sample Types: Whole Cells
Applications: ICCC Blume et al. (2021-01-11), A novel ACE2 isoform is expressed in human respiratory epithelia and is upregulated in response to interferons and RNA respiratory virus infection Nature Genetics, 2021-00(0).
PMID: 33432184 -
Species: Mesocricetus auratus (Hamster)
Sample Types: Whole Cells
Applications: Flow CytometryH Chu et al. (2021-01-08), Host and viral determinants for efficient SARS-CoV-2 infection of the human lung Nature Communications, 2021-012(1):134.
PMID: 33420022 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryF Onodi et al. (2021-01-08), SARS-CoV-2 induces human plasmacytoid pre-dendritic cell diversification via UNC93B and IRAK4 bioRxiv : the preprint server for biology, 2021-00(0).
PMID: 33442685 -
Species: Human
Sample Types: Whole Tissue,Whole Cells
Applications: IHC,ICCAZ Mykytyn et al. (2021-01-04), SARS-CoV-2 entry into human airway organoids is serine protease-mediated and facilitated by the multibasic cleavage site Elife, 2021-010(0).
PMID: 33393462 -
Species: Human
Sample Types: Organoid
Applications: ICCRM Samuel et al. (2020-11-17), Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men Cell Stem Cell, 2020-127(6):876-889.e12.
PMID: 33232663 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotJK Osan et al. (2020-11-12), Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in COPD bioRxiv, 2020-10(0).
PMID: 33200131 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotR Tandon et al. (2020-11-05), Effective screening of SARS-CoV-2 neutralizing antibodies in patient serum using lentivirus particles pseudotyped with SARS-CoV-2 spike glycoprotein Sci Rep, 2020-110(1):19076.
PMID: 33154514 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCIT Lee et al. (2020-10-28), ACE2 localizes to the respiratory cilia and is not increased by ACE inhibitors or ARBs Nat Commun, 2020-111(1):5453.
PMID: 33116139 -
Species: Human
Sample Types: Whole Cells
Applications: ICCW Rut et al. (2020-10-22), SARS-CoV-2 Mpro inhibitors and activity-based probes for patient-sample imaging Nat Chem Biol, 2020-10(0).
PMID: 33093684 -
Species: Primate
Sample Types: Whole Cells
Applications: Neutralization,ICCX Xie et al. (2020-10-15), A nanoluciferase SARS-CoV-2 for rapid neutralization testing and screening of anti-infective drugs for COVID-19 Nat Commun, 2020-111(1):5214.
PMID: 33060595 -
Species: Human
Sample Types: Protein
Applications: Western BlotL Casciola-R et al. (2020-10-15), IgM autoantibodies recognizing ACE2 are associated with severe COVID-19 medRxiv, 2020-10(0).
PMID: 33083808 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCM Baratchian et al. (2020-10-14), Sex, androgens and regulation of pulmonary AR, TMPRSS2 and ACE2 bioRxiv, 2020-10(0).
PMID: 33083800 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotL Pellegrini et al. (2020-10-13), SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids Cell Stem Cell, 2020-10(0).
PMID: 33113348 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCSP Sajuthi et al. (2020-10-12), Type 2 and interferon inflammation regulate SARS-CoV-2 entry factor expression in the airway epithelium Nat Commun, 2020-111(1):5139.
PMID: 33046696 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotR Wang et al. (2020-09-24), Functional genomic screens identify human host factors for SARS-CoV-2 and common cold coronaviruses bioRxiv : the preprint server for biology, 2020-00(0).
PMID: 32995787 -
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,ICCJ Huang et al. (2020-09-18), SARS-CoV-2 Infection of Pluripotent Stem Cell-Derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response Cell Stem Cell, 2020-00(0).
PMID: 32979316 -
Species: Mouse,Human
Sample Types: Whole Tissue,Whole Cells
Applications: IHC,NeutralizationF Duan et al. (2020-09-15), Modeling COVID-19 with Human Pluripotent Stem Cell-Derived Cells Reveals Synergistic Effects of Anti-inflammatory Macrophages with ACE2 Inhibition Against SARS-CoV-2 Res Sq, 2020-00(0).
PMID: 32839764 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryI Busnadiego et al. (2020-09-10), Antiviral Activity of Type I, II, and III Interferons Counterbalances ACE2 Inducibility and Restricts SARS-CoV-2 MBio, 2020-011(5).
PMID: 32913009 -
Species: Human
Sample Types: Organoid
Applications: NeutralizationE Song et al. (2020-09-08), Neuroinvasion of SARS-CoV-2 in human and mouse brain bioRxiv : the preprint server for biology, 2020-00(0).
PMID: 32935108 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotT Azad et al. (2020-08-30), Implications for SARS-CoV-2 Vaccine Design: Fusion of Spike Glycoprotein Transmembrane Domain to Receptor-Binding Domain Induces Trimerization Membranes (Basel), 2020-010(9).
PMID: 32872641 -
Species: Human
Sample Types: Whole Cells
Applications: ICC,Flow CytometryJ Huang et al. (2020-08-06), SARS-CoV-2 Infection of Pluripotent Stem Cell-derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response bioRxiv, 2020-00(0).
PMID: 32637964 -
Species: Human
Sample Types: Whole Cells
Applications: ICCJ Prévost et al. (2020-07-30), Cross-sectional evaluation of humoral responses against SARS-CoV-2 Spike bioRxiv : the preprint server for biology, 2020-00(0).
PMID: 32577637 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryR Rathnasing et al. (2020-07-06), Comparison of Transgenic and Adenovirus hACE2 Mouse Models for SARS-CoV-2 Infection bioRxiv, 2020-00(0).
PMID: 32676603 -
Species: Human
Sample Types: Whole Tissue,Tissue Homogenates
Applications: IHC,Western BlotJ Collin et al. (2020-06-03), Co-expression of SARS-CoV-2 entry genes in the superficial adult human conjunctival, limbal and corneal epithelium suggests an additional route of entry via the ocular surface Ocul Surf, 2020-00(0).
PMID: 32502616 -
Species: Bat,Human
Sample Types: Whole Cells
Applications: ICCJ Zhou et al. (2020-05-13), Infection of bat and human intestinal organoids by SARS-CoV-2 Nat. Med., 2020-00(0).
PMID: 32405028 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCIT Lee et al. (2020-05-12), Robust ACE2 protein expression localizes to the motile cilia of the respiratory tract epithelia and is not increased by ACE inhibitors or angiotensin receptor blockers medRxiv, 2020-00(0).
PMID: 32511516 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryKHD Crawford et al. (2020-05-06), Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays Viruses, 2020-012(5).
PMID: 32384820 -
Species: Human
Sample Types: Whole Cells
Applications: ICCY Han et al. (2020-05-05), Identification of Candidate COVID-19 Therapeutics using hPSC-derived Lung Organoids bioRxiv, 2020-00(0).
PMID: 32511403 -
Species: Mouse,Human
Sample Types: Cell Lysates,Tissue Homogenates
Applications: Western BlotM Yang et al. (2020-04-08), Liraglutide Attenuates Non-Alcoholic Fatty Liver Disease in Mice by Regulating the Local Renin-Angiotensin System Front Pharmacol, 2020-011(0):432.
PMID: 32322207 -
Species: Primate - Chlorocebus aethiops (African Green Monkey)
Sample Types: Whole Cells
Applications: NeutralizationHoffmann M et al. (2020-03-05), SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell, 2020-0181(0):1-10.
PMID: 32142651 -
Species: Rhesus Macaca (Macaque)
Sample Types: Whole Tissue
Applications: IHC-PD Tian et al. (2019-06-24), Protective effects of the Francisella tularensis δpdpC mutant against its virulent parental strain SCHU P9 in Cynomolgus macaques Sci Rep, 2019-09(1):9193.
PMID: 31235714 -
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: Western Blot,Flow CytometryE Schrom et al. (2017-04-13), Translation of Angiotensin-Converting Enzyme 2 upon Liver- and Lung-Targeted Delivery of Optimized Chemically Modified mRNA Mol Ther Nucleic Acids, 2017-07(0):350-365.
PMID: 28624211 -
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western BlotFengxia Xiao et al. (2016-06-01), Protein Kinase C-? Mediates Shedding of Angiotensin-Converting Enzyme 2 from Proximal Tubular Cells. Frontiers in Pharmacology, 2016-00(0):1663-9812.
PMID: 27313531 -
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: IHCAleksandra Milewska (2016-06-01), HTCC: Broad Range Inhibitor of Coronavirus Entry PLoS ONE, 2016-011(6):e0156552.
PMID: 27249425 -
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: NeutralizationYu Y et al. (2016-01-22), Surface vimentin is critical for the cell entry of SARS-CoV. J Biomed Sci, 2016-023(1):14.
PMID: 26801988 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotXiao F et al. (2014-01-15), Characterization of angiotensin-converting enzyme 2 ectodomain shedding from mouse proximal tubular cells. PLoS ONE, 2014-09(1):e85958.
PMID: 24454948 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCEckerle I et al. (2013-12-23), In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection. Virol J, 2013-110(0):359.
PMID: 24364985 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryHeurich A et al. (2013-11-13), TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein. J Virol, 2013-188(2):1293-307.
PMID: 24227843 -
Species: Bat,Human,Civet
Sample Types: Whole Cells
Applications: IHCGe et al. (2013-10-30), Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor. Nature, 2013-1503(7477):535-8.
PMID: 24172901 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PBertram S et al. (2012-04-30), Influenza and SARS-coronavirus activating proteases TMPRSS2 and HAT are expressed at multiple sites in human respiratory and gastrointestinal tracts. PLoS ONE, 2012-07(4):e35876.
PMID: 22558251 -
Species: Mouse
Sample Types: Whole Tissue,Tissue Homogenates
Applications: Western Blot,IHC-FrPohl M et al. (2010-10-21), Intrarenal renin angiotensin system revisited: role of megalin-dependent endocytosis along the proximal nephron. J. Biol. Chem., 2010-1285(53):41935-46.
PMID: 20966072 -
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Cell Lysates
Applications: Western BlotGlende J et al. (2008-09-23), Importance of cholesterol-rich membrane microdomains in the interaction of the S protein of SARS-coronavirus with the cellular receptor angiotensin-converting enzyme 2. Virology, 2008-0381(2):215-21.
PMID: 18814896 -
Species: Human
Sample Types: Plasma
Applications: NeutralizationEpelman S et al. (2008-08-26), Detection of soluble angiotensin-converting enzyme 2 in heart failure: insights into the endogenous counter-regulatory pathway of the renin-angiotensin-aldosterone system. J. Am. Coll. Cardiol., 2008-052(9):750-4.
PMID: 18718423 -
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: Flow CytometryLu Y et al. (2008-02-13), Lipid rafts are involved in SARS-CoV entry into Vero E6 cells. Biochem. Biophys. Res. Commun., 2008-0369(2):344-9.
PMID: 18279660 -
Species: Human
Sample Types: Whole Cells,Cell Lysates
Applications: ICC,Western BlotRen W et al. (2007-12-12), Difference in receptor usage between severe acute respiratory syndrome (SARS) coronavirus and SARS-like coronavirus of bat origin. J. Virol., 2007-182(4):1899-907.
PMID: 18077725 -
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Cell Lysates
Applications: Western BlotInoue Y et al. (2007-05-23), Clathrin-dependent entry of severe acute respiratory syndrome coronavirus into target cells expressing ACE2 with the cytoplasmic tail deleted. J. Virol., 2007-081(16):8722-9.
PMID: 17522231 -
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: NeutralizationRockx B et al. (2007-05-16), Synthetic reconstruction of zoonotic and early human severe acute respiratory syndrome coronavirus isolates that produce fatal disease in aged mice. J. Virol., 2007-081(14):7410-23.
PMID: 17507479 -
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: ICCde Lang A et al. (2006-07-24), Interferon-gamma and interleukin-4 downregulate expression of the SARS coronavirus receptor ACE2 in Vero E6 cells. Virology, 2006-0353(2):474-81.
PMID: 16860835 -
Species: Human
Sample Types: Whole Cells
Applications: ICC,NeutralizationTseng CT et al. (2005-08-01), Apical entry and release of severe acute respiratory syndrome-associated coronavirus in polarized Calu-3 lung epithelial cells. J. Virol., 2005-079(15):9470-9.
PMID: 16014910 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryHofmann H et al. (2004-07-09), Susceptibility to SARS coronavirus S protein-driven infection correlates with expression of angiotensin converting enzyme 2 and infection can be blocked by soluble receptor. Biochem. Biophys. Res. Commun., 2004-0319(4):1216-21.
PMID: 15194496
There are no citations that match your criteria.
Customer Reviews for Human/Mouse/Rat/Hamster ACE‑2 Antibody (4)
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Application: Block/NeutralizeSample Tested: Embryonic lungSpecies: HumanVerified Customer | Posted 10/04/2022
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Stimulated Human Bronchial Epithelial Cell Line (BEAS-2B)Species: HumanVerified Customer | Posted 03/09/2021
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Application: Western BlotSample Tested: Human CD34+ Cells and Blood mononuclear cells (PBMCs)Species: HumanVerified Customer | Posted 08/12/2016
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Application: ImmunofluorescenceSample Tested: See PMID 23232719Species: HumanVerified Customer | Posted 01/12/2015
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