Key Product Details
Product Summary for Human/Mouse/Rat/Hamster ACE‑2 Antibody
Accession # Q9BYF1
Scientific Data Images for Human/Mouse/Rat/Hamster ACE‑2 Antibody
Detection of Human, Mouse, and Rat ACE‑2 by Western Blot.Western blot shows lysates of human kidney tissue, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ACE-2 at approximately 100 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry.HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF933) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). Quadrant markers were set based on Goat IgG control antibody (AB-108-C, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.
ACE‑2 in Human Kidney.ACE-2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Applications for Human/Mouse/Rat/Hamster ACE‑2 Antibody
Blockade of Receptor-ligand Interaction
Sample: HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP
Sample: Immersion fixed frozen sections of rat lung, immersion fixed paraffin-embedded sections of human kidney, hamster lung, rat lung, and rat kidney
Sample: Conditioned cell culture medium spiked with Recombinant Human ACE‑2 (Catalog # 933-ZN), see our available Western blot detection antibodies
Sample: Human kidney tissue
Sample: Human kidney tissue, Mouse kidney tissue, and Rat kidney tissue
Read 4 reviews rated 5 using AF933 in the following applications:
- Block/Neutralize (1 Review)
- Immunocytochemistry/Immunofluorescence (1 Review)
- Immunofluorescence (1 Review)
- Western Blot (1 Review)
Read 117 publications using AF933 in the following applications:
- Co-Immunoprecipitation (2 Publications)
- Control (1 Publication)
- ELISA Detection (1 Publication)
- FLOW (1 Publication)
- Flow Cytometry (32 Publications)
- ICC (26 Publications)
- IHC (28 Publications)
- IHC-Fr (1 Publication)
- IHC-P (2 Publications)
- Neutralization (15 Publications)
- Western Blot (37 Publications)
Formulation, Preparation and Storage
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).
ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (4, 5). This Human anti-ACE2 antibody (catalog # AF933) was used to block the variant SARS-CoV-2 and ACE2 interaction to elucidate viral transmission and potential therapeutic strategies. (5)
- Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
- Crackower, M.A. et al. (2002) Nature 417:822.
- Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
- Li, W. et al. (2003) Nature 426:450.
- Hoffmann, M. et al. (2020) Cell. DOI: 10.1016/j.cell.2020.02.052.
Entrez Gene IDs
Product Specific Notices for Human/Mouse/Rat/Hamster ACE‑2 Antibody
For research use only