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Human/Mouse/Rat/Hamster ACE‑2 Antibody Best Seller

Catalog # AF933 | R&D Systems, Inc. a Bio-Techne Brand
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AF933
AF933-SP

Key Product Details

Species Reactivity

Human, Mouse, Rat, Hamster

Applications

Blockade of Receptor-ligand Interaction, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human/Mouse/Rat/Hamster ACE‑2 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ACE-2
Gln18-Ser740
Accession # Q9BYF1

Specificity

Detects human ACE-2 in direct ELISAs. Detects human, mouse, and rat ACE-2 in Western blots. Detects Hamster ACE-2 in immunohistochemistry.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat/Hamster ACE‑2 Antibody

Detection of Human, Mouse, and Rat ACE‑2 antibody by Western Blot.

Detection of Human, Mouse, and Rat ACE‑2 by Western Blot.

Western blot shows lysates of human kidney tissue, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ACE-2 at approximately 100 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of ACE-2 antibody in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP antibody by Flow Cytometry.

Detection of ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF933) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). Quadrant markers were set based on Goat IgG control antibody (AB-108-C, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.
ACE-2 antibody in Human Kidney by Immunohistochemistry (IHC-P).

ACE‑2 in Human Kidney.

ACE-2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse/Rat/Hamster ACE‑2 Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

Hoffman, M. et al. (2020) Cell. DOI: 10.1016/j.cell.2020.02.052. This application was not tested by R&D Systems.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed frozen sections of rat lung, immersion fixed paraffin-embedded sections of human kidney, hamster lung, rat lung, and rat kidney

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ACE‑2 (Catalog # 933-ZN), see our available Western blot detection antibodies

Simple Western

10 µg/mL
Sample: Human kidney tissue

Western Blot

1 µg/mL
Sample: Human kidney tissue, Mouse kidney tissue, and Rat kidney tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 5 using AF933 in the following applications:

Published Applications

Read 117 publications using AF933 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ACE-2

ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).

ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (4, 5). This Human anti-ACE2 antibody (catalog # AF933) was used to block the variant SARS-CoV-2 and ACE2 interaction to elucidate viral transmission and potential therapeutic strategies. (5)

References

  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower,  M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
  4. Li, W. et al. (2003) Nature 426:450.
  5. Hoffmann, M. et al. (2020) Cell. DOI: 10.1016/j.cell.2020.02.052.

Long Name

Angiotensin I Converting Enzyme 2

Alternate Names

ACE2, ACEH

Entrez Gene IDs

480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 59272 (Human); 108390919 (Malayan Pangolin); 70008 (Mouse); 100144303 (Porcine); 302668 (Rat)

Gene Symbol

ACE2

UniProt

Product Documents for Human/Mouse/Rat/Hamster ACE‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat/Hamster ACE‑2 Antibody

For research use only

Citations for Human/Mouse/Rat/Hamster ACE‑2 Antibody (117)

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Customer Reviews for Human/Mouse/Rat/Hamster ACE‑2 Antibody (4)

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  • Human/Mouse/Rat/Hamster ACE-2 Antibody
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: Embryonic lung
    Species: Human
    Verified Customer | Posted 10/04/2022
    Human/Mouse/Rat/Hamster ACE‑2 Antibody AF933
  • Human/Mouse/Rat/Hamster ACE-2 Antibody
    Name: Yuchen Liu
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Stimulated Human Bronchial Epithelial Cell Line (BEAS-2B)
    Species: Human
    Verified Customer | Posted 03/09/2021
    Human/Mouse/Rat/Hamster ACE‑2 Antibody AF933
  • Human ACE-2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human CD34+ Cells and Blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 08/12/2016
    Human/Mouse/Rat/Hamster ACE‑2 Antibody AF933
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23232719
    Species: Human
    Verified Customer | Posted 01/12/2015

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