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Key Product Details

Species Reactivity

Human, Mouse, Rat, Hamster

Applications

Immunohistochemistry, Western Blot, Blockade of Receptor-ligand Interaction, Flow Cytometry, Immunoprecipitation

Label

Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ACE-2
Gln18-Ser740
Accession # Q9BYF1

Specificity

Detects human ACE-2 in direct ELISAs. Detects human, mouse, and rat ACE-2 in Western blots. Detects Hamster ACE-2 in immunohistochemistry.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ACE-2

ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).

ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (4, 5). This Human anti-ACE2 antibody (catalog # AF933) was used to block the variant SARS-CoV-2 and ACE2 interaction to elucidate viral transmission and potential therapeutic strategies. (5)

Long Name

Angiotensin I Converting Enzyme 2

Alternate Names

ACE2, ACEH

Entrez Gene IDs

59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)

Gene Symbol

ACE2

UniProt

Additional ACE-2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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