Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52644CL1
Recombinant Monoclonal Antibody.
![Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/nbp2-52644cl1_rabbit-fc-gamma-rii-riii-cd32-cd16-mab-2-4g2-cl1-129202514393314.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready
Label
CoraFluor 1
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 2.4G2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse BALB/c Macrophage J774 Cell Line.
Specificity
This antibody binds to an epitope on the extracellular domains of the mouse Fc(gamma)III and Fc(gamma)II, thereby inhibiting their ability to bind antibodies via their Fc region.
receptors. Also binding the Fc(gamma)I receptor (CD64) via its Fc domain.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Scientific Data Images for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [CoraFluor™ 1]
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Fc gamma RII/RIII (CD32/CD16)
Fc gamma RII/CD32 and Fc gamma RIII/CD16 proteins are low/intermediate affinity receptors for polyvalent immune-complexed IgG. Fc gamma RII is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Fc gamma RIIA associates with FcR gamma and delivers an activating signal through its cytoplasmic ITAM motif. In contrast, Fc gamma RIIB delivers an inhibitory signal through its ITIM motif. Fc gamma RIIC represents an unequal cross-over event between the IIA and IIB genes. Fc gamma RIII is expressed on NK cells, T cells, monocytes, and macrophages. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes.
Long Name
Fc gamma Receptor II/III
Alternate Names
CD32/CD16
Gene Symbol
FCGR3A
Additional Fc gamma RII/RIII (CD32/CD16) Products
Product Documents for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [CoraFluor™ 1]
Product Specific Notices for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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