Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [mFluor Violet 500 SE]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52644MFV500
Recombinant Monoclonal Antibody
![CD16/CD32 Antibody (2.4G2) [mFluor Violet 500 SE]](https://resources.bio-techne.com/images/products/nbp2-52644mfv500_rabbit-cd16-cd32-mab-2-4g2-mfluor-violet-500-se-57202313435789.png "CD16/CD32 Antibody (2.4G2) [mFluor Violet 500 SE] -")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready
Label
mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 2.4G2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse BALB/c Macrophage J774 Cell Line.
Specificity
This antibody binds to an epitope on the extracellular domains of the mouse Fc(gamma)III and Fc(gamma)II, thereby inhibiting their ability to bind antibodies via their Fc region.
receptors. Also binding the Fc(gamma)I receptor (CD64) via its Fc domain.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [mFluor Violet 500 SE]
CD16/CD32 Antibody (2.4G2) [mFluor Violet 500 SE] -
CD16/CD32 Antibody (2.4G2) [mFluor Violet 500 SE] - Vial of mFluor Violet 500 conjugated antibody. mFluor Violet 500 is optimally excited at 410 nm by the Violet laser (405 nm) and has an emission maximum of 501 nm.Applications for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [mFluor Violet 500 SE]
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP2-52644. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Fc gamma RII/RIII (CD32/CD16)
Fc gamma RII/CD32 and Fc gamma RIII/CD16 proteins are low/intermediate affinity receptors for polyvalent immune-complexed IgG. Fc gamma RII is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Fc gamma RIIA associates with FcR gamma and delivers an activating signal through its cytoplasmic ITAM motif. In contrast, Fc gamma RIIB delivers an inhibitory signal through its ITIM motif. Fc gamma RIIC represents an unequal cross-over event between the IIA and IIB genes. Fc gamma RIII is expressed on NK cells, T cells, monocytes, and macrophages. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes.
Long Name
Fc gamma Receptor II/III
Alternate Names
CD32/CD16
Gene Symbol
FCGR3A
Additional Fc gamma RII/RIII (CD32/CD16) Products
Product Documents for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [mFluor Violet 500 SE]
Product Specific Notices for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [mFluor Violet 500 SE]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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