Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [PE/Cy5.5]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52644PECY55
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, Product Image
Label
PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 2.4G2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse BALB/c Macrophage J774 Cell Line.
Reactivity Notes
Mouse
Specificity
This antibody binds to an epitope on the extracellular domains of the mouse Fc(gamma)III and Fc(gamma)II, thereby inhibiting their ability to bind antibodies via their Fc region.
receptors. Also binding the Fc(gamma)I receptor (CD64) via its Fc domain.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [PE/Cy5.5]
Product Image: CD16/CD32 Antibody (2.4G2) [PE/Cy5.5] [NBP2-52644PECY55] - Vial of PE/Cy5.5 conjugated antibody. PE/Cy5.5 has two excitation maxima, 498 nm excited by the Blue laser (488 nm) and 566 nm excited by the Yellow-Green laser (561 nm). Both result in emission at 700 nm.
Applications for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [PE/Cy5.5]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Product Image
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Fc gamma RII/RIII (CD32/CD16)
Fc gamma RII/CD32 and Fc gamma RIII/CD16 proteins are low/intermediate affinity receptors for polyvalent immune-complexed IgG. Fc gamma RII is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Fc gamma RIIA associates with FcR gamma and delivers an activating signal through its cytoplasmic ITAM motif. In contrast, Fc gamma RIIB delivers an inhibitory signal through its ITIM motif. Fc gamma RIIC represents an unequal cross-over event between the IIA and IIB genes. Fc gamma RIII is expressed on NK cells, T cells, monocytes, and macrophages. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes.
Long Name
Fc gamma Receptor II/III
Alternate Names
CD32/CD16
Gene Symbol
FCGR3A
Additional Fc gamma RII/RIII (CD32/CD16) Products
Product Documents for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [PE/Cy5.5]
Product Specific Notices for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [PE/Cy5.5]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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