Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [Alexa Fluor™ Plus 555] - Chimeric
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52644AFP555
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready
Label
Alexa Fluor Plus 555
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 2.4G2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse BALB/c Macrophage J774 Cell Line.
Specificity
This antibody binds to an epitope on the extracellular domains of the mouse Fc(gamma)III and Fc(gamma)II, thereby inhibiting their ability to bind antibodies via their Fc region.
receptors. Also binding the Fc(gamma)I receptor (CD64) via its Fc domain.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Applications
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Fc gamma RII/RIII (CD32/CD16)
Fc gamma RII/CD32 and Fc gamma RIII/CD16 proteins are low/intermediate affinity receptors for polyvalent immune-complexed IgG. Fc gamma RII is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Fc gamma RIIA associates with FcR gamma and delivers an activating signal through its cytoplasmic ITAM motif. In contrast, Fc gamma RIIB delivers an inhibitory signal through its ITIM motif. Fc gamma RIIC represents an unequal cross-over event between the IIA and IIB genes. Fc gamma RIII is expressed on NK cells, T cells, monocytes, and macrophages. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes.
Long Name
Fc gamma Receptor II/III
Alternate Names
CD32/CD16
Gene Symbol
FCGR3A
Additional Fc gamma RII/RIII (CD32/CD16) Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...