CoraFluor™ 2, amine reactive
Tocris Bioscience, part of Bio-Techne | Catalog # 7950
Terbium cryptate FRET donor for TR-FRET assay development; amine reactive for conjugation
Key Product Details
Description
Terbium cryptate FRET donor for TR-FRET assay development; amine reactive for conjugation
Product Description
CoraFluor™ 2, amine reactive is a terbium-based time-resolved Förster resonance energy transfer (TR-FRET) donor for TR-FRET assay development, such as target engagement and ternary complex assays. CoraFluor™ 2 is designed with a PFP ester for reactivity with primary amines, making it suitable for labeling biomolecules, including antibodies, nanobodies, proteins and peptides. CoraFluor™ 2 is cell permeable and can be used in live cell intracellular TR-FRET assays. It is exceptionally bright, providing unmatched assay sensitivity and stability in biological media over other commercially available TR-FRET donors. CoraFluor™ 2 displays red shifted excitation and is excitable by both 365 nm and 405 nm lasers, and is compatible with common acceptor dyes such as FAM/FITC, AF488, GFP and mCherry, enabling seamless assay integration in high throughput screening applications.CoraFluor™ 1, amine reactive, CoraFluor™ 1, thiol reactive, CoraFluor™ 1, Haloalkane, and CoraFluor™ 2, Haloalkane, also available.
Please refer to the protocol for further information on conjugation.
For more information, please see our CoraFluor™ TR-FRET technology flyer .
CoraFluor™ is a trademark of Bio-Techne Corp.
Scientific Data Images for CoraFluor™ 2, amine reactive
CoraFluor™ 2 stability profile.
Stability profiling of CoraFluor™ 2 and a commercially available TR-FRET donor (5 nM) after 7 days of exposure to 50 mM PBS, acetic acid (pH 3), sodium acetate (pH 5), MES (pH 6) or Tris buffer (pH 7.5) supplemented with various additives (DTPA, Ca2+, Mg2+, DTT, TCEP = 1 mM; Mn2+, Cu2+ = 0.5 mM).
Live cell TR-FRET assay.
Cellular dose-dependent inhibition of HDAC1 via CoraFluor™ 2 labeled HaloTag® in HEK293T cells by various HDAC inhibitors (treatment for 4 h at 37 °C, 1 μM SAHA-NCT). HaloTag is a trademark of Promega Corporation.Licensing Information
Sold for research purposes only under agreement from Massachusetts General Hospital, US Patent No. 12,134,721. A license is required for development of new commercial assay platforms (e.g., microarrays, microfluidics), diagnostic use or other unlicensed uses. Please contact Technical Support for further details: techsupport@bio-techne.com.Optical Data
| λabs | 352 nm |
| Reactivity | Primary amines |
| Application | Time-resolved fluorescence energy transfer (TR-FRET) |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Product Specifications
Molecular Weight
1623.02
Storage
Store at -20°C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Calculators
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Background References
References are publications that support the biological activity of the product.
- Payne Bright and stable luminescent probes for target engagement profiling in live cells. Nat.Chem.Biol. 2021 PMID: 34675420
Product Specific Notices for CoraFluor™ 2, amine reactive
For research use only
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