FITC
Tocris Bioscience, part of Bio-Techne | Catalog # 5440
Green fluorescent dye
Key Product Details
Description
Green fluorescent dye
Alternative Names
Fluorescein-5-Isothiocyanate,FITC Isomer 1
Product Description
Key information: FITC (an amine-reactive version of the parent dye, fluorescein) is a widely-used green fluorescent dye supplied with an isothiocyanate reactive group for the labeling of primary amines. FITC is bright and has good water-solubility, however it is sensitive to changes in pH and temperature, and has a relatively high photobleaching rate.Application: Suitable for flow cytometry, immunofluorescence (IHC, ICC), fluorescence microscopy, fluorescence in situ hybridization (FISH), biochemical assays.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 495 nm and 525 nm, respectively; quantum yield = 0.92; extinction coefficient = 75,000 M-1cm-1; A280 = 0.35.
Optical Data
| Emission Color | Green |
| Brightness | |
| λabs | 495 nm |
| λem | 525 nm |
| Extinction Coefficient (ε) | 75000 M-1cm-1 |
| Quantum Yield (φ) | 0.92 |
| Closest Laser line | 488 nm |
| Reactive Group | Isothiocyanate |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.35 |
| Application | Flow Cytometry, Immunofluorescence (IHC, ICC), Fluorescence Microscopy, FISH |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Product Specifications
Molecular Weight
389.38
Formula
C21H11NO5S
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
3',6'-Dihydroxy-5-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
CAS Number
3326-32-7
PubChem ID
18730
InChI Key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
SMILES
OC1=CC=C(C2(C(C=CC(N=C=S)=C3)=C3C(O2)=O)C(C=CC(O)=C4)=C4O5)C5=C1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 38.94 | 100 |
Preparing Stock Solutions
for FITC
The following data is based on the product molecular weight 389.38
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.57 mL | 12.84 mL | 25.68 mL |
| 5 mM | 0.51 mL | 2.57 mL | 5.14 mL |
| 10 mM | 0.26 mL | 1.28 mL | 2.57 mL |
| 50 mM | 0.05 mL | 0.26 mL | 0.51 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Yang A novel CXCR4-selective high-affinity fluorescent probe and its application in competitive binding assays. Biochemistry 2014 PMID: 25058910
Product Specific Notices for FITC
For research use only
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