GDP-Biotin-Fucose
R&D Systems, part of Bio-Techne | Catalog # ES202
Key Product Details
Key Benefits
Learn more about Fluorescent Glycan Labeling and Detection
Species
Multi-Species
Conjugate
Biotin
Product Summary for GDP-Biotin-Fucose
|
Formula |
C37H57N10O21P2S |
|
Molecular Weight |
1085.93 Da |
|
Formulation |
Lyophilized with Tris, at pH 8.0 |
|
Stability & Storage |
Store the unopened product at ≤ -20 °C. Use a manual defrost freezer and aviod repeated freeze-thaw cycles. Good for 12 months from date of receipt. |
Applications
- Biotinylation of free glycans as well as glycoproteins and glycolipids.
- Detect specific glycan epitopes on glycoproteins as well as cell surface.
Key Features and Benefits:
- Can be introduced to proteins and lipids via various fucosyltransferases.
- Can be introduced to live cells for imaging.
- Minimum side effect on target molecules.
- Convenient and User-friendly.
For Details:
Wu, ZL. et al., (2020) Glycobiology 30:970.
Related reagents
Click Chemistry
- GDP-Cy5-Fucose (ES301)
- CMP-Cy5-Sialic Acid (ES302)
- GDP-Azido-Fucose (ES101)
- CMP-Azido-Sialic Acid (ES102)
- UDP-Azido-GalNAc (ES103)
- UDP-Azido-GlcNAc (ES104)
- CMP-9-Biotin-Sialic Acid (ES201)
Enzymes and Detection Reagents for GDP-Biotin-Fucose, ES202
- Various Fucosyltransferases
- Streptavidin-HRP (DY998)
Data Examples
Table 1. Enzyme selection for Glycan Labeling
| Fucosyltransferase | R&D Systems, Catalog # | Substrate | GDP-Biotin-Fucose Tolerance |
| Fucosyltransferase 1/FUT1 | Terminal Gal in H antigen | Not determined | |
| Fucosyltransferase 2/FUT2 | 7770-GT | Terminal Gal in H antigen | Yes |
| Fucosyltransferase 3/FUT3 | 4950-GT | GlcNAc in type 1 glycan chain | Yes |
| Fucosyltransferase 4/FUT4 | GlcNAc in terminal lactosamine | Yes | |
| Fucosyltransferase 5/FUT5 | 4949-GT | GlcNAc in terminal lactosamine | Yes |
| Fucosyltransferase 6/FUT6 | GlcNAc in terminal lactosamine | Yes | |
| Fucosyltransferase 7/FUT7 | 6409-GT | GlcNAc in sialylated lactosamine | Yes |
| Fucosyltransferase 8/FUT8 | 5768-GT | Core GlcNAc in N-glycans | Yes |
| Fucosyltransferase 9/FUT9 | 9347-GT | GlcNAc in non-sialylated lactosamine | Yes |
| Fucosyltransferase 10/FUT10 | Unknown | Unknown | |
| Fucosyltransferase 11/FUT11 | 5964-GT | Unknown | Unknown |
| Recombinant Human Protein O-fucosyltransferase 1/POFUT1, CF | 7409-GT | Notch receptor | Not determined |
Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at < -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Good for 12 months from date of receipt.
Product Documents for GDP-Biotin-Fucose
Product Specific Notices for GDP-Biotin-Fucose
For research use only
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