Spike RBD Antibody (1035753) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # FAB105808CL1
Detects SARS-CoV-2 Spike RBD and Spike S1 in sandwich ELISAs
![Spike RBD Antibody (1035753) [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/fab105808cl1_mouse-spike-rbd-mab-1035753-corafluor-1-26920251730204.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Key Product Details
Species Reactivity
SARS-CoV-2
Applications
ELISA, Blockade of Receptor-ligand Interaction
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 Clone # 1035753
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Spodoptera frugiperda, Sf 21 (baculovirus)-derived SARS-CoV-2 Spike S1 Subunit
Val16-Pro681
Accession # YP_009724390.1
Val16-Pro681
Accession # YP_009724390.1
Specificity
In a functional flow cytometry assay, this antibody binds and blocks SARS-CoV-2 Spike RBD interaction with human ACE-2 transfectants. Detects SARS-CoV-2 Spike RBD and SARS-CoV-2 Spike S1 in sandwich ELISAs when used in the indicated ELISA antibody pairings. Detects SARS-CoV-2 B.1.1.529 S RBD (Omicron Variant) in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Scientific Data Images for Spike RBD Antibody (1035753) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for Spike RBD Antibody (1035753) [CoraFluor™ 1]
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Spike RBD
The coronavirus Spike protein receptor binding domain (RBD) resides within the S1 subunit and is responsible for binding to host cell receptors and initiating viral infection. Past coronaviruses SARS and MERS along with the global pandemic caused by SARS-CoV-2 have sparked great interest and scientific discovery leading to new vaccines and drug development. At the heart of coronavirus biology lies the Spike protein and its receptor binding domain. The Spike RBD is a 26 kDa domain consisting of a twisted five-stranded antiparallel beta sheet and sits at the apex of each Spike protein monomer. The Spike RBD is flexible thanks to a hinge region that allows for conformational changes that expose (up or open conformation) or hide (down or closed conformation) its receptor contacts. For SARS-CoV-2, the Spike RBD recognizes and tightly binds to the human receptor ACE-2. In the closely related MERS coronavirus, human DPPIV/CD26 acts as the receptor.
Long Name
Spike Receptor Binding Domain
Additional Spike RBD Products
Product Documents for Spike RBD Antibody (1035753) [CoraFluor™ 1]
Product Specific Notices for Spike RBD Antibody (1035753) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...