MDGA1 Antibody [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # AF5055CL1
![MDGA1 Antibody [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/af5055cl1_sheep-mdga1-pab-corafluor-1-81020251239530.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
CoraFluor 1
Antibody Source
Polyclonal Sheep IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MDGA1
Gln19-Gly931
Accession # Q8NFP4
Gln19-Gly931
Accession # Q8NFP4
Specificity
Detects human MDGA1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human MDGA2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Scientific Data Images for MDGA1 Antibody [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for MDGA1 Antibody [CoraFluor™ 1]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: MDGA1
MDGA-1 (MAM domain-containing GPI anchor protein 1) is a 135-140 kDa, novel member of the Ig-superfamily. It is expressed on embryonic neurons destined for cortical layers 2/3, as well as migrating basilar pontine neurons and D1 interneurons of the spinal cord. Human proMDGA-1 is 937 amino acids (aa) in length (SwissProt #:Q8NFP4). It is a GPI-linked glycoprotein that contains six consecutive Ig-like domains (aa #24-631), followed by one fibronectin type III domain (aa #640-739), a MAM region (aa #751-918), and a cleavable propeptide (aa #933-955). There are at least three potential variants. One shows a 48 aa substitution for aa #936-955, a second shows a 58 aa substitution for aa #847-955, and a third shows a 51 aa substitution for aa #537-955. Over aa #19-931, human MDGA-1 is 95% aa identical to mouse MDGA-1.
Long Name
MAM Domain Containing Glycosylphosphatidylinositol Anchor 1
Alternate Names
GPIM, MAMDC3
Gene Symbol
MDGA1
Additional MDGA1 Products
Product Documents for MDGA1 Antibody [CoraFluor™ 1]
Product Specific Notices for MDGA1 Antibody [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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