HEK293 human embryonic kidney cell line transfected with either (A) human CHRM5 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human CHRM5 Monoclonal Antibody (Catalog # MAB10323) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CHRM5 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human CHRM5 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CHRM5

Muscarinic receptors are widely distributed throughout the body and control distinct functions according to location and subtype (M1 - M5). They are predominantly expressed in the parasympathetic nervous system where they exert both inhibitory and excitatory effects. Stimulation of CHRM5 is known to increase cyclic AMP levels. The clinical implications of CHRM5 are unknown but it may be linked to schizophrenia.

Long Name

Cholinergic Receptor Muscarinic 5

Alternate Names

HM5, M5, M5R, mAChR M5

Entrez Gene IDs

1133 (Human); 213788 (Mouse); 53949 (Rat)

Gene Symbol

CHRM5

UniProt

P08912

Additional CHRM5 Products

Product Documents for Human CHRM5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CHRM5 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CHRM5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10323

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CHRM5 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CHRM5 Antibody

Detection of CHRM5 in HEK293 Human Cell Line Transfected with Human CHRM5 and eGFP by Flow Cytometry.