Mouse IgG2A Isotype Control Best Seller
Catalog # MAB003 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Mouse IgG2A Isotype Control
Immunogen
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse IgG2A Isotype Control
Detection of Mouse IgG Control by Flow Cytometry
Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).Applications for Mouse IgG2A Isotype Control
Control
Reviewed Applications
Read 8 reviews rated 4.4 using MAB003 in the following applications:
- Block/Neutralize CONTROL (1 Review)
- Block/Neutralize (1 Review)
- Flow Cytometry (3 Reviews)
- Western Blot (1 Review)
Published Applications
Read 65 publications using MAB003 in the following applications:
- Bioassay (1 Publication)
- Control (19 Publications)
- Electrophysiology (1 Publication)
- Flow Cytometry (7 Publications)
- Flow Cytometry Control (1 Publication)
- Functional Assay (1 Publication)
- ICC (2 Publications)
- IHC (2 Publications)
- IHC Control (1 Publication)
- IHC-P (2 Publications)
- Immunoprecipitation (1 Publication)
- In Vivo (1 Publication)
- Isotype Control (8 Publications)
- Neutralization (9 Publications)
- Neutralization Control (9 Publications)
Formulation, Preparation and Storage
Purification
Endotoxin Level
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG2A
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Alternate Names
Product Documents for Mouse IgG2A Isotype Control
Product Specific Notices for Mouse IgG2A Isotype Control
For research use only
Citations for Mouse IgG2A Isotype Control (65)
Citations are publications that use Bio-Techne products. Selected citations for Mouse IgG2A Isotype Control include:
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Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryKoh et al. (2023-06-01), Antitumor effects of NK cells expanded by activation pre?processing of autologous feeder cells before irradiation in colorectal cancer Oncology letters
PMID: 37153058 -
Species: Human
Sample Types: Whole Cells
Applications: Neutralization ControlBacke et al. (2023-05-25), PhosY-secretome profiling combined with kinase-substrate interaction screening defines active c-Src-driven extracellular signaling Cell reports
PMID: 37243593 -
Species: Human
Sample Types: Whole Cells
Applications: Isotype ControlM Oba et al. (2023-03-21), CCR7 Mediates Cell Invasion and Migration in Extrahepatic Cholangiocarcinoma by Inducing Epithelial-Mesenchymal Transition Cancers, 2023-015(6).
PMID: 36980764 -
Species: Human
Sample Types: Whole Cells
Applications: Neutralization ControlXY Cui et al. (2022-11-08), FSAP Protects against Histone-Mediated Increase in Endothelial Permeability In Vitro International Journal of Molecular Sciences, 2022-123(22).
PMID: 36430180 -
Species: Rat
Sample Types: In Vivo
Applications: In VivoY Morita et al. (2022-10-27), A novel aquaporin-4-associated optic neuritis rat model with severe pathological and functional manifestations Journal of Neuroinflammation, 2022-119(1):263.
PMID: 36303157 -
Species: Mouse
Sample Types: In Vivo
R Chimienti et al. (2022-09-27), Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement Cell Reports, 2022-040(13):111423.
PMID: 36170817 -
Species: Mouse
Sample Types: Whole Tissue
Applications: Isotype ControlJF de Graaf et al. (2022-07-09), Comparison between intratumoral and intravenously administered oncolytic virus therapy with Newcastle disease virus in a xenograft murine model for pancreatic adenocarcinoma Heliyon, 2022-08(7):e09915.
PMID: 35874055 -
Species: Duck
Sample Types: Whole Tissue
Applications: IHC ControlACM de Bruin et al. (2022-01-17), Reduced Replication of Highly Pathogenic Avian Influenza Virus in Duck Endothelial Cells Compared to Chicken Endothelial Cells Is Associated with Stronger Antiviral Responses Viruses, 2022-014(1).
PMID: 35062369 -
Species: Human
Sample Types: Whole Cells
Applications: Neutralization ControlS Mairpady S et al. (2022-01-10), Hyperinflammatory environment drives dysfunctional myeloid cell effector response to bacterial challenge in COVID-19 PloS Pathogens, 2022-018(1):e1010176.
PMID: 35007290 -
Species: Mouse
Sample Types: Whole Tissue
Applications: ElectrophysiologyM Di Filippo et al. (2021-12-07), Interleukin-17 affects synaptic plasticity and cognition in an experimental model of multiple sclerosis Cell Reports, 2021-137(10):110094.
PMID: 34879272 -
Species: Human
Sample Types: Whole Cells
Applications: Neutralization ControlD Maura et al. (2021-07-08), The ammonia oxidizing bacterium Nitrosomonas eutropha blocks T helper 2 cell polarization via the anti-inflammatory cytokine IL-10 Scientific Reports, 2021-011(1):14162.
PMID: 34238943 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryI Trenevska et al. (2021-04-09), Comprehensive mutagenesis identifies the peptide repertoire of a p53 T-cell receptor mimic antibody that displays no toxicity in mice transgenic for human HLA-A*0201 PLoS ONE, 2021-016(4):e0249967.
PMID: 33836029 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationFY Mao et al. (2021-03-03), Helicobacter pylori-induced Rev-erb&alpha fosters gastric bacteria colonization by impairing host innate and adaptive defense Cellular and Molecular Gastroenterology and Hepatology, 2021-00(0).
PMID: 33676046 -
Species: Mouse
Sample Types: Serum
Applications: Isotype ControlS Salari et al. (2021-02-18), The immunity of the recombinant prokaryotic and eukaryotic subunit vaccines against cutaneous leishmaniasis Microbial pathogenesis, 2021-0153(0):104807.
PMID: 33609648 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationX Chen et al. (2021-01-19), ILT4 inhibition prevents TAM- and dysfunctional T cell-mediated immunosuppression and enhances the efficacy of anti-PD-L1 therapy in NSCLC with EGFR activation Theranostics, 2021-011(7):3392-3416.
PMID: 33537094 -
Species: Human
Sample Types: Whole Cells
Applications: Isotype ControlRV Broad et al. (2021-01-04), Inhibition of interferon-signalling halts cancer-associated fibroblast-dependent protection of breast cancer cells from chemotherapy British Journal of Cancer, 2021-00(0).
PMID: 33398063 -
Species: Human
Sample Types: Whole Cells
Applications: Neutralization ControlX Li et al. (2020-11-09), IL35 predicts prognosis in gastric cancer and is associated with angiogenesis by altering TIMP1, PAI1, and IGFBP1 FEBS Open Bio, 2020-10(0).
PMID: 33064893 -
Species: Mouse
Sample Types: In Vivo
Applications: NeutralizationRJ Napier et al. (2020-10-26), T cell-intrinsic role for Nod2 in protection against Th17-mediated uveitis Nat Commun, 2020-111(1):5406.
PMID: 33106495 -
Species: Human
Sample Types: Whole Cells
Applications: Neutralization ControlX Ji et al. (2020-03-23), Nfa34810 Facilitates Nocardia farcinica Invasion of Host Cells and Stimulates Tumor Necrosis Factor Alpha Secretion through Activation of the NF-&kappaB and Mitogen-Activated Protein Kinase Pathways via Toll-Like Receptor 4 Infect. Immun., 2020-088(4).
PMID: 31964749 -
Species: Human
Sample Types: Whole Cells
Applications: Neutralization ControlR Kumar et al. (2020-02-25), Type I Interferons Suppress Anti-parasitic Immunity and Can Be Targeted to Improve Treatment of Visceral Leishmaniasis Cell Rep, 2020-030(8):2512-2525.e9.
PMID: 32101732 -
Species: Mouse
Sample Types: Cell Culture Lysates
Applications: ImmunoprecipitationM Kobayashi et al. (2020-02-19), Proteome Profiling Uncovers an Autoimmune Response Signature That Reflects Ovarian Cancer Pathogenesis Cancers (Basel), 2020-012(2).
PMID: 32092936 -
Species: Ferret
Sample Types: Whole Tissue
Applications: IHCM Richard et al. (2020-02-07), Influenza A viruses are transmitted via the air from the nasal respiratory epithelium of ferrets Nat Commun, 2020-011(1):766.
PMID: 32034144 -
Species: Mouse
Sample Types: Whole Cells
Applications: Isotype ControlN Inagaki-Ka et al. (2019-07-11), Statins can suppress dendritic cell-mediated Th2 responses through the repression of OX40-ligand and CCL17 expression Eur. J. Immunol., 2019-00(0).
PMID: 31269241 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PA Stepanjuk et al. (2019-05-11), MUC20 expression marks the receptive phase of the human endometrium Reprod. Biomed. Online, 2019-00(0).
PMID: 31519421 -
Species: Human
Sample Types: Whole Cells
Applications: ICCH Okada et al. (2019-03-18), Function and Immunogenicity of Gene-corrected iPSC-derived Hepatocyte-Like Cells in Restoring Low Density Lipoprotein Uptake in Homozygous Familial Hypercholesterolemia Sci Rep, 2019-09(1):4695.
PMID: 30886174 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationZ Bánki et al. (2019-02-25), Antibody opsonization enhances MAIT cell responsiveness to bacteria via a TNF dependent mechanism Immunol. Cell Biol., 2019-00(0).
PMID: 30695101 -
Species: Rat
Sample Types: Whole Cells
Applications: Flow CytometrySM Koura et al. (2019-02-01), Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration Mol Med Rep, 2019-00(0).
PMID: 30720108 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryS Tamura et al. (2018-01-20), Molecular Correlates ofIn VitroResponses to Dacomitinib and Afatinib in Bladder Cancer Bladder Cancer, 2018-04(1):77-90.
PMID: 29430509 -
Species: Primate
Sample Types: Whole Tissue
Applications: IHC-PT Kuiken et al. (2017-11-21), Pigeon paramyxovirus type 1 from a fatal human case induces pneumonia in experimentally infected cynomolgus macaques (Macaca fascicularis) Vet. Res., 2017-148(1):80.
PMID: 29162154 -
Species: Human
Sample Types: Whole Cells
Applications: ControlYE Du et al. (2017-09-15), MiR-205/YAP1 in Activated Fibroblasts of Breast Tumor Promotes VEGF-independent Angiogenesis through STAT3 Signaling Theranostics, 2017-07(16):3972-3988.
PMID: 29109792 -
Applications: Neutralization ControlAK Reuschl et al. (2017-09-01), Innate activation of human primary epithelial cells broadens the host response to Mycobacterium tuberculosis in the airways PLoS Pathog., 2017-013(9):e1006577.
PMID: 28863187 -
Species: Human
Sample Types: Whole Cells
Applications: ICCF Anfasa et al. (2017-07-26), Phenotypic Differences between Asian and African Lineage Zika Viruses in Human Neural Progenitor Cells mSphere, 2017-02(4).
PMID: 28815211 -
Species: Human
Sample Types: Whole Tissue,Whole Cells
Applications: Flow Cytometry Control,IHCA Llibre et al. (2016-12-29), Expression of lectin-like transcript-1 in human tissues F1000Res, 2016-15(0):2929.
PMID: 28413611 -
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization(2016-12-09), EphA4 may contribute to microvessel remodeling in the hippocampal CA1 and CA3 areas in a mouse model of temporal lobe epilepsy Mol Med Rep, 2016-10(0).
PMID: 27959424 -
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization(2016-11-22), A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10 Sci Rep, 2016-15(0):37366.
PMID: 27874028 -
Species: Human
Sample Types: Whole Cells
Applications: ControlWan-Chien Cheng (2016-08-01), Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses Eur J Immunol, 2016-00(0).
PMID: 27334899 -
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry(2016-05-09), USP6 oncogene promotes Wnt signaling by deubiquitylating Frizzleds Proc Natl Acad Sci USA, 2016-0113(21):E2945-54.
PMID: 27162353 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayZou Y et al. (2015-08-04), NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure. PLoS ONE, 2015-010(8):e0134568.
PMID: 26241657 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationGiese et al. (2014-07-03), Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells. PLoS Pathog, 2014-010(7):e1004189.
PMID: 24991932 -
Species: Mouse
Sample Types: In Vivo
Applications: Isotype ControlNagai et al. (2014-05-20), Spontaneous CNV in a novel mutant mouse is associated with early VEGF-A-driven angiogenesis and late-stage focal edema, neural cell loss, and dysfunction. Invest Ophthalmol Vis Sci, 2014-055(6):3709-19.
PMID: 24845632 -
Species: Human
Sample Types: Whole Blood
Applications: Isotype ControlRobinet P et al. (2014-04-30), A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells. J Immunol, 2014-0192(11):5332-42.
PMID: 24790151 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayPicco G et al. (2014-04-17), Targeting DNGR-1 (CLEC9A) with antibody/MUC1 peptide conjugates as a vaccine for carcinomas. Eur J Immunol, 2014-044(7):1947-55.
PMID: 24648154 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometrySrivastava et al. (2014-04-07), Type I interferons directly inhibit regulatory T cells to allow optimal antiviral T cell responses during acute LCMV infection. J Exp Med, 2014-0211(5):961-74.
PMID: 24711580 -
Species: Mouse
Sample Types: In Vivo
Applications: NeutralizationKlein M et al. (2014-04-03), Leukocyte attraction by CCL20 and its receptor CCR6 in humans and mice with pneumococcal meningitis. PLoS ONE, 2014-09(4):e93057.
PMID: 24699535 -
Species: Human
Sample Types: Whole Cells
Applications: ControlMoller-Tank S et al. (2014-04-02), Characterizing functional domains for TIM-mediated enveloped virus entry. J Virol, 2014-088(12):6702-13.
PMID: 24696470 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryHuijbers I et al. (2014-01-08), Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells. EMBO Mol Med, 2014-06(2):212-25.
PMID: 24401838 -
Species: Human
Sample Types: Whole Cells
Applications: Isotype ControlRybak A et al. (2013-04-19), Propagation of human prostate cancer stem-like cells occurs through EGFR-mediated ERK activation. PLoS ONE, 2013-08(4):e61716.
PMID: 23620784 -
Species: Human
Sample Types: Whole Cells
Applications: Neutralization ControlStone et al. (2013-04-18), Hepatitis C virus pathogen associated molecular pattern (PAMP) triggers production of lambda-interferons by human plasmacytoid dendritic cells. PLoS Pathog, 2013-09(4):e1003316.
PMID: 23637605 -
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization(2013-01-23), Mesothelial cells give rise to hepatic stellate cells and myofibroblasts via mesothelial-mesenchymal transition in liver injury. Proc Natl Acad Sci U S A, 2013-0110(6):2324-9.
PMID: 23345421 -
Species: Human
Sample Types: Whole Cells
Applications: ControlBenito-Miguel M et al. (2012-07-09), IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival. PLoS ONE, 2012-07(7):e40620.
PMID: 22792388 -
Species: Human
Sample Types: Whole Cells
Applications: ControlNielsen N et al. (2012-02-22), Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A. PLoS ONE, 2012-07(2):e31959.
PMID: 22384114 -
Species: Human
Sample Types: Whole Cells
Applications: ControlBin L et al. (2011-07-14), Inhibition of transcription factor specificity protein 1 alters the gene expression profile of keratinocytes leading to upregulation of kallikrein-related peptidases and thymic stromal lymphopoietin. J. Invest. Dermatol., 2011-0131(11):2213-22.
PMID: 21753780 -
Species: Mouse
Sample Types: In Vivo
Applications: ControlBarlow JL et al. (2011-07-04), Reciprocal expression of IL-25 and IL-17A is important for allergic airways hyperreactivity. Clin. Exp. Allergy, 2011-041(10):1447-55.
PMID: 21722219 -
Species: Human
Sample Types: Whole Cells
Applications: ControlReikvam H et al. (2009-11-17), Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation. Eur. J. Haematol., 2009-184(3):239-51.
PMID: 19922462 -
Species: Human
Sample Types: Whole Cells
Applications: ControlShiozawa Y et al. (2009-11-14), GAS6/Mer axis regulates the homing and survival of the E2A/PBX1-positive B-cell precursor acute lymphoblastic leukemia in the bone marrow niche. Exp. Hematol., 2009-138(2):132-40.
PMID: 19922767 -
Species: Mouse
Sample Types: In Vivo
Applications: ControlChauhan SK et al. (2009-02-01), Autoimmunity in dry eye is due to resistance of Th17 to Treg suppression. J. Immunol., 2009-0182(3):1247-52.
PMID: 19155469 -
Species: Human
Sample Types: Whole Cells
Applications: ControlInglefield JR et al. (2008-04-01), TLR7 Agonist 852A Inhibition of Tumor Cell Proliferation Is Dependent on Plasmacytoid Dendritic Cells and Type I IFN. J. Interferon Cytokine Res., 2008-028(4):253-63.
PMID: 18439103 -
Species: Human
Sample Types: Whole Cells
Applications: ControlWeindl G et al. (2007-12-01), Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling. J. Clin. Invest., 2007-1117(12):3664-3672.
PMID: 17992260 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: ControlDuluc D et al. (2007-09-11), Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells. Blood, 2007-0110(13):4319-30.
PMID: 17848619 -
Species: Human
Sample Types: Whole Cells
Applications: ControlArnold R et al. (2007-05-01), Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells. Immunology, 2007-0121(1):71-81.
PMID: 17425601 -
Species: Human
Sample Types: Whole Tissue
Applications: ControlBhandari V et al. (2006-11-05), Hyperoxia causes angiopoietin 2-mediated acute lung injury and necrotic cell death. Nat. Med., 2006-112(11):1286-93.
PMID: 17086189 -
Species: Human
Sample Types: Whole Cells
Applications: ControlSmith C et al. (2006-09-27), Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation. J. Am. Coll. Cardiol., 2006-048(8):1591-9.
PMID: 17045893 -
Species: Human
Sample Types: Whole Cells
Applications: ControlYu LC et al. (2006-06-22), LPS/CD14 activation triggers SGLT-1-mediated glucose uptake and cell rescue in intestinal epithelial cells via early apoptotic signals upstream of caspase-3. Exp. Cell Res., 2006-0312(17):3276-86.
PMID: 16860318 -
Species: Human
Sample Types: Whole Cells
Applications: ControlGounni Abdelilah S et al. (2006-06-16), Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006-0120(2):220-31.
PMID: 16782408 -
Species: Human
Sample Types: Whole Cells
Applications: ControlTraves SL et al. (2004-05-20), Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2. J. Leukoc. Biol., 2004-076(2):441-50.
PMID: 15155777
There are no citations that match your criteria.
Customer Reviews for Mouse IgG2A Isotype Control (8)
Customer Images
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Application: Block/NeutralizeSample Tested: LAD2 human mast cellsSpecies: HumanVerified Customer | Posted 04/07/2022
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Application: Flow CytometrySample Tested: Bone ExtractsSpecies: HumanVerified Customer | Posted 02/13/2020
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Application: Block/Neutralize CONTROLSample Tested: Peripheral blood T cellsSpecies: HumanVerified Customer | Posted 01/08/2020
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Sample Tested: Bone ExtractsSpecies: HumanVerified Customer | Posted 09/10/2019Need optimization of your specific cell types and use the same amount as the primary antibody. Saw lots of binding for the isotype control.
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Application: Western BlotSample Tested: Recombinant proteinSpecies: MouseVerified Customer | Posted 08/04/2019
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Application: Flow CytometrySample Tested: Blood mononuclear cells (PBMCs)Species: HumanVerified Customer | Posted 03/23/2018
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Verified Customer | Posted 06/13/2017
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Application: Flow CytometrySample Tested: NCI-H146Species: HumanVerified Customer | Posted 08/03/2016NCI-H146 cells stained with test antibody (blue) or isotype control (red) at 10 ug/ml and then stained with secondary PE antibody.
There are no reviews that match your criteria.
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