Human CD57 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11708
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1088014
Product Specifications
Immunogen
Whole Cell
Accession # Q9P2W7
Accession # Q9P2W7
Specificity
Detects the CD57 carbohydrate epitope in Human PBMCs
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CD57 Antibody
Detection of CD57 in PBMC by Flow Cytometry
PBMC were stained with Rabbit Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB24086P) and either (A) Mouse Anti-Human CD57 Monoclonal Antibody (Catalog # MAB11708) or (B) isotype control antibody (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Detection of CD57 in Human Lymph Node.
CD57 was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human CD57 Monoclonal Antibody (Catalog # MAB11708) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of CD57 in Human Spleen.
CD57 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CD57 Monoclonal Antibody (Catalog # MAB11708) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human CD57 Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: Human Peripheral Blood Mononuclear Cells (PBMCs)
Sample: Human Peripheral Blood Mononuclear Cells (PBMCs)
Immunohistochemistry
1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node, spleen and tonsil
Sample: Immersion fixed paraffin-embedded sections of human lymph node, spleen and tonsil
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD57
References
1. Kared H, Martelli S, Ng TP, Pender SL, Larbi A. CD57 in human
natural killer cells and T-lymphocytes. Cancer Immunol Immunother. 2016
Apr;65(4):441-52. doi: 10.1007/s00262-016-1803-z. Epub 2016 Feb 5. PMID:
26850637; PMCID: PMC11029668.
2. Elias Junior E, Gubert VT, Bonin-Jacob CM, Puga MAM, Gouveia
CG, Sichinel AH, Tozetti IA. CD57 T cells associated with immunosenescence in
adults living with HIV or AIDS. Immunology. 2024 Jan;171(1):146-153. doi:
10.1111/imm.13707. Epub 2023 Oct 25. PMID: 37880915.
3. Lian J, Yue Y, Yu W, Zhang Y. Immunosenescence: a key player
in cancer development. J Hematol Oncol. 2020 Nov 10;13(1):151. doi:
10.1186/s13045-020-00986-z. Erratum in: J Hematol Oncol. 2025 Mar 5;18(1):25.
doi: 10.1186/s13045-025-01682-6. PMID: 33168037; PMCID: PMC7653700.
Long Name
Cluster of Differentiation 57
Alternate Names
Human Natural Killer-1, L2, LEU-7
Gene Symbol
B3GAT1
UniProt
Additional CD57 Products
Product Documents for Human CD57 Antibody
Product Specific Notices for Human CD57 Antibody
For research use only
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