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Human CD3 epsilon Antibody Best Seller
Catalog # MAB100 | R&D Systems, Inc. a Bio-Techne Brand
Clone UCHT1 was used by HLDA to establish CD designation
Key Product Details
Species Reactivity
Human
Applications
CyTOF-reported, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, T Cell Stimulation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # UCHT1
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Product Summary for Human CD3 epsilon Antibody
Immunogen
Human thymocytes (1)
Specificity
Recognizes the epsilon-chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD3 epsilon Antibody
Detection of Human CD3 epsilon by Western Blot.
Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line (negative control), and THP‑1 human acute monocytic leukemia cell line (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD3 epsilon at approximately 21 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
CD3 epsilon in Human PBMCs.
CD3e was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).Applications for Human CD3 epsilon Antibody
Application
Recommended Usage
CyTOF-reported
Behbehani, G.K. et al. (2015) Cancer Discov. 5: 988. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood lymphocytes
Sample: Human peripheral blood lymphocytes
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Immunoprecipitation
McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York
T Cell Stimulation
This antibody can be used to activate T cells when immobilized at 1-10 μg/mL (100 μL/well).
Western Blot
2 µg/mL
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 5 using MAB100 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD3 epsilon
CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
Product Documents for Human CD3 epsilon Antibody
Product Specific Notices for Human CD3 epsilon Antibody
For research use only
Citations for Human CD3 epsilon Antibody (56)
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Citations are publications that use Bio-Techne products. Selected citations for Human CD3 epsilon Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: StimulationD Peruzzu et al. (2022-08-15), Anti-Inflammatory Effects of 1,25(OH)2D/Calcitriol in T Cell Immunity: Does Sex Make a Difference? International Journal of Molecular Sciences, 2022-023(16).
PMID: 36012424 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayL Yang et al. (2022-03-26), Overexpression of ascitic interleukin-35 induces CD8+ T cell exhaustion in liver cirrhotic patients with spontaneous bacterial peritonitis International immunopharmacology, 2022-0108(0):108729.
PMID: 35349961 -
Species: Primate
Sample Types: Whole Tissue
Applications: IHCRM Gopalakris et al. (2021-10-22), Increased IL-6 expression precedes reliable viral detection in the rhesus macaque brain during acute SIV infection JCI Insight, 2021-16(20).
PMID: 34676832 -
Species: Human
Sample Types: Whole Cells
Applications: ELISA DevelopmentMC Cavé et al. (2020-07-02), Glycosaminoglycans bind human IL-27 and regulate its activity Eur. J. Immunol., 2020-00(0).
PMID: 32483835 -
Species: Human
Sample Types: Whole Cells
Applications: T Cell Activation,Functional AssayJ Huetter et al. (2020-04-20), Characterization of BAY 1905254, an Immune Checkpoint Inhibitor Targeting the Immunoglobulin-Like Domain Containing Receptor 2 (ILDR2) Cancer Immunol Res, 2020-08(7):895-911.
PMID: 32312711 -
Species: Human
Sample Types: Whole Tissue
Applications: Tissue CultureML Tiirikaine et al. (2019-12-27), Ex vivo culture of lesional psoriasis skin for pharmacological testing J. Dermatol. Sci., 2019-10(0).
PMID: 31948839 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayJC Chamcheu et al. (2019-09-15), Fisetin, a 3,7,3',4'-Tetrahydroxyflavone Inhibits the PI3K/Akt/mTOR and MAPK Pathways and Ameliorates Psoriasis Pathology in 2D and 3D Organotypic Human Inflammatory Skin Models Cells, 2019-08(9).
PMID: 31540162 -
Species: Human
Sample Types: Whole Cells
Applications: StimulationNB Zhou et al. (2019-08-16), Effect of morphine and a low dose of ketamine on the T cells of patients with refractory cancer pain in vitro Oncol Lett, 2019-018(4):4230-4236.
PMID: 31516618 -
Species: Human
Sample Types: Whole Cells
Applications: StimulationMA Dragovich et al. (2019-06-14), SLAMF6 clustering is required to augment T cell activation PLoS ONE, 2019-014(6):e0218109.
PMID: 31199820 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryN Beha et al. (2019-04-30), IL-15-based trifunctional antibody-fusion proteins with costimulatory TNF-superfamily ligands in the single-chain format for cancer immunotherapy Mol. Cancer Ther., 2019-00(0).
PMID: 31040163 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayF Bensch et al. (2018-11-26), 89Zr-atezolizumab imaging as a non-invasive approach to assess clinical response to PD-L1 blockade in cancer Nat. Med., 2018-10(0).
PMID: 30478423 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayR Fischer et al. (2017-07-26), Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2 Sci Rep, 2017-07(1):6607.
PMID: 28747780 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryC Caër et al. (2017-06-07), Immune cell-derived cytokines contribute to obesity-related inflammation, fibrogenesis and metabolic deregulation in human adipose tissue Sci Rep, 2017-07(1):3000.
PMID: 28592801 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayS Nakachi et al. (2017-05-16), Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis Arthritis Res. Ther., 2017-019(1):97.
PMID: 28511719 -
Species: Human
Sample Types: Whole Cells
Applications: ICCM Strazza et al. (2017-02-17), PLC?1 regulates SDF-1?-induced lymphocyte adhesion and migration to sites of inflammation Proc. Natl. Acad. Sci. U.S.A, 2017-00(0).
PMID: 28213494 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayC Tao et al. (2017-02-15), ??TCR immunoglobulin constant region domain exchange in human ??TCRs improves TCR pairing without altering TCR gene-modified T cell function Mol Med Rep, 2017-00(0).
PMID: 28259946 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCElena Neumann (2016-11-22), Expression of adipokines in osteoarthritis osteophytes and their effect on osteoblasts Matrix Biol., 2016-10(0).
PMID: 27884778 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryXun Qu (2015-06-01), Human trophoblast cells induced MDSCs from peripheral blood CD14(+) myelomonocytic cells via elevated levels of CCL2 Cell. Mol. Immunol., 2015-013(5):615-27.
PMID: 26027727 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayHuang W et al. (2015-05-21), T-type calcium channel antagonists, mibefradil and NNC-55-0396 inhibit cell proliferation and induce cell apoptosis in leukemia cell lines. J Exp Clin Cancer Res, 2015-034(0):54.
PMID: 25989794 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayPatsoukis N et al. (2015-03-26), PD-1 alters T-cell metabolic reprogramming by inhibiting glycolysis and promoting lipolysis and fatty acid oxidation. Nat Commun, 2015-06(0):6692.
PMID: 25809635 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayMoore C et al. (2014-12-10), Direct and indirect effects of immune and central nervous system-resident cells on human oligodendrocyte progenitor cell differentiation. J Immunol, 2014-1194(2):761-72.
PMID: 25505283 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryVan Belle T et al. (2014-10-03), 1,25-Dihydroxyvitamin D3 and its analog TX527 promote a stable regulatory T cell phenotype in T cells from type 1 diabetes patients. PLoS ONE, 2014-19(10):e109194.
PMID: 25279717 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayFan et al. (2014-09-24), Optimizations of siRNA design for the activation of gene transcription by targeting the TATA-box motif. PLoS ONE, 2014-09(9):e108253.
PMID: 25250958 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayZhu L et al. (2014-06-27), Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation. J Thromb Haemost, 2014-012(7):1156-65.
PMID: 24833264 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryGao D et al. (2014-04-03), Autologous tumor lysate-pulsed dendritic cell immunotherapy with cytokine-induced killer cells improves survival in gastric and colorectal cancer patients. PLoS ONE, 2014-09(4):e93886.
PMID: 24699863 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayRuan M et al. (2014-03-21), The secretion of IL-6 by CpG-ODN-treated cancer cells promotes T-cell immune responses partly through the TLR-9/AP-1 pathway in oral squamous cell carcinoma. Int J Oncol, 2014-044(6):2103-10.
PMID: 24676671 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayTodaro M et al. (2014-03-06), CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis. Cell Stem Cell, 2014-014(3):342-56.
PMID: 24607406 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssaySgarbanti M et al. (2014-01-06), IkappaB kinase epsilon targets interferon regulatory factor 1 in activated T lymphocytes. Mol Cell Biol, 2014-034(6):1054-65.
PMID: 24396068 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayRenand A et al. (2013-12-30), Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs. PLoS ONE, 2013-18(12):e85589.
PMID: 24386482 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayCevikbas F et al. (2013-12-25), A sensory neuron-expressed IL-31 receptor mediates T helper cell-dependent itch: Involvement of TRPV1 and TRPA1. J Allergy Clin Immunol, 2013-1133(2):448-60.
PMID: 24373353 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayWang et al. (2013-09-03), Regulated capture by exosomes of mRNAs for cytoplasmic tRNA synthetases. J Biol Chem, 2013-0288(41):29223-8.
PMID: 24003230 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayRamgolam VS et al. (2011-03-02), B cells as a therapeutic target for IFN-beta in relapsing-remitting multiple sclerosis. J. Immunol., 2011-0186(0):4518.
PMID: 21368231 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayEikawa S et al. (2010-11-03), Enrichment of Foxp3+ CD4 regulatory T cells in migrated T cells to IL-6- and IL-8-expressing tumors through predominant induction of CXCR1 by IL-6. J. Immunol., 2010-1185(11):6734-40.
PMID: 21048114 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayJones GW et al. (2010-09-08), Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation. FASEB J., 2010-025(1):409-19.
PMID: 20826539 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayPennino D et al. (2010-03-31), IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes. J. Immunol., 2010-0184(9):4880-8.
PMID: 20357258 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayWitte K et al. (2009-10-01), Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines. Genes Immun., 2009-110(8):702-14.
PMID: 19798076 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayNi Z et al. (2009-08-08), Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation. Clin. Immunol., 2009-0133(2):257-64.
PMID: 19665434 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayLongo O et al. (2009-02-07), Phase I therapeutic trial of the HIV-1 Tat protein and long term follow-up. Vaccine, 2009-027(25):3306-12.
PMID: 19208456 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayLundqvist A et al. (2009-02-06), Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells. Blood, 2009-0113(24):6120-7.
PMID: 19202127 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayHirosako S et al. (2008-11-26), Human bronchial intraepithelial T cells produce interferon-gamma and stimulate epithelial cells. Clin. Exp. Immunol., 2008-1155(2):266-74.
PMID: 19040600 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr,IHC-PTran CN et al. (2008-03-01), Interactions of T cells with fibroblast-like synoviocytes: role of the B7 family costimulatory ligand B7-H3. J. Immunol., 2008-0180(5):2989-98.
PMID: 18292521 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayMcFadden C et al. (2007-11-15), Preferential migration of T regulatory cells induced by IL-16. J. Immunol., 2007-1179(10):6439-45.
PMID: 17982032 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayRossol M et al. (2007-09-15), Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells. J. Immunol., 2007-0179(6):4239-48.
PMID: 17785864 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayStebbings R et al. (2007-09-01), "Cytokine storm" in the phase I trial of monoclonal antibody TGN1412: better understanding the causes to improve preclinical testing of immunotherapeutics. J. Immunol., 2007-0179(5):3325-31.
PMID: 17709549 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayBayry J et al. (2007-04-01), Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells. J. Immunol., 2007-0178(7):4184-93.
PMID: 17371975 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayMamoni RL et al. (2006-10-11), Flow-cytometric analysis of cytokine production in human paracoccidioidomycosis. Cytokine, 2006-135(3):207-16.
PMID: 17045486 -
Species: Human
Sample Types: Whole Tissue,Whole Cells
Applications: Immunoprecipitation,IHC-FrKawai T et al. (2006-09-01), B and T lymphocytes are the primary sources of RANKL in the bone resorptive lesion of periodontal disease. Am. J. Pathol., 2006-0169(3):987-98.
PMID: 16936272 -
Species: Human
Applications: Binding AssayMarkovic SN et al. (2006-09-01), A reproducible method for the enumeration of functional (cytokine producing) versus non-functional peptide-specific cytotoxic T lymphocytes in human peripheral blood. Clin. Exp. Immunol., 2006-0145(3):438-47.
PMID: 16907911 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayRen Y et al. (2005-06-01), Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis. Histopathology, 2005-046(6):659-67.
PMID: 15910597 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayOgilvie RL et al. (2005-01-15), Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay. J. Immunol., 2005-0174(2):953-61.
PMID: 15634918 -
Species: Human
Sample Types: Whole Cells
Applications: ICCLiao TS et al. (2005-01-01), Recruitment of osteoclast precursors by stromal cell derived factor-1 (SDF-1) in giant cell tumor of bone. J. Orthop. Res., 2005-023(1):203-9.
PMID: 15607894 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayWolk K et al. (2004-08-01), IL-22 increases the innate immunity of tissues. Immunity, 2004-021(2):241-54.
PMID: 15308104 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayWang P et al. (2004-06-01), Induction of lysosomal and plasma membrane-bound sialidases in human T-cells via T-cell receptor. Biochem. J., 2004-0380(0):425-33.
PMID: 14992689 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayJanardhan A et al. (2004-01-20), HIV-1 Nef binds the DOCK2-ELMO1 complex to activate rac and inhibit lymphocyte chemotaxis. PLoS Biol., 2004-02(1):E6.
PMID: 14737186 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayTakahashi R et al. (2003-12-01), In vitro differentiation from naive to mature E-selectin binding CD4 T cells: acquisition of skin-homing properties occurs independently of cutaneous lymphocyte antigen expression. J. Immunol., 2003-1171(11):5769-77.
PMID: 14634085 -
Species: Feline
Sample Types: Whole Cells
Applications: Flow CytometryHan MK et al. (2003-04-01), NF-kappaB-dependent lymphocyte hyperadhesiveness to synovial fibroblasts by hypoxia and reoxygenation: potential role in rheumatoid arthritis. J. Leukoc. Biol., 2003-073(4):525-9.
PMID: 12660227
There are no citations that match your criteria.
Customer Reviews for Human CD3 epsilon Antibody (4)
5 out of 5
4 customer
ratings
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Application: ImmunohistochemistrySample Tested: Spleen tissueSpecies: MouseVerified Customer | Posted 04/18/2018
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Application: ImmunohistochemistrySample Tested: Tumor cell lyastesSpecies: HumanVerified Customer | Posted 04/13/2018
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Application: Flow CytometrySample Tested: Blood mononuclear cells (PBMCs)Species: HumanVerified Customer | Posted 03/21/2018
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Application: ELISASample Tested: Human recombinant antibodySpecies: HumanVerified Customer | Posted 10/25/2017
There are no reviews that match your criteria.
FAQs for Human CD3 epsilon Antibody
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Q: What is the protocol used to immobilize MAB100 for T cell stimulation?What is the protocol used to immobilize MAB100 for T cell stimulation?
A: The protocol is based on the following reference: J. Immunology. (1992) 149:3448-3455.
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Q: What is the optimal working concentration of the antibody?
A: Novus' product datasheets provide a recommended concentration for each validated application that is a starting point for titration. The concentration required will vary from lab to lab due to other variables and thus titration is strongly recommended for optimizing results.
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