Human/Mouse/Rat GAPDH Antibody
Catalog # MAB5718 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Human/Mouse/Rat GAPDH Antibody
Immunogen
Met1-Ala150
Accession # P04406
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse/Rat GAPDH Antibody
Detection of Human, Mouse, and Rat GAPDH/G3PDH by Western Blot.
Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF Membrane was probed with 0.05 µg/mL of Mouse Anti-Human/Mouse/Rat GAPDH/G3PDH Monoclonal Antibody (Catalog # MAB5718) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GAPDH/G3PDH at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.GAPDH in HeLa Human Cell Line.
GAPDH was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat GAPDH Monoclonal Antibody (Catalog # MAB5718) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.GAPDH/G3PDH in Human Kidney.
GAPDH/G3PDH was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat GAPDH/G3PDH Monoclonal Antibody (Catalog # MAB5718) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse/Rat GAPDH Antibody
Immunocytochemistry
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Immunohistochemistry
Sample: Immersion fixed paraffin-embedded sections of human kidney
Simple Western
Sample: Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line
Western Blot
Sample: Human brain tissue, mouse brain tissue, and rat brain tissue
Reviewed Applications
Read 3 reviews rated 5 using MAB5718 in the following applications:
Published Applications
Read 17 publications using MAB5718 in the following applications:
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GAPDH
aa 1‑150, human GAPDH shares 92% aa identity with mouse GAPDH.
Product Documents for Human/Mouse/Rat GAPDH Antibody
Product Specific Notices for Human/Mouse/Rat GAPDH Antibody
For research use only
Citations for Human/Mouse/Rat GAPDH Antibody (17)
Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat GAPDH Antibody include:
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Species: Human
Sample Types: Cell Lysates
Applications: Western BlotS Mohammadho et al. (2023-01-07), Arachidin-1, a Prenylated Stilbenoid from Peanut, Enhances the Anticancer Effects of Paclitaxel in Triple-Negative Breast Cancer Cells Cancers, 2023-015(2).
PMID: 36672351 -
H Kim et al. (2022-08-05), Exosome-guided direct reprogramming of tumor-associated macrophages from protumorigenic to antitumorigenic to fight cancer Bioactive materials, 2022-025(0):527-540.
PMID: 37056267 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotF Schneider et al. (2021-06-17), Functional Redundancy of Cyclase-Associated Proteins CAP1 and CAP2 in Differentiating Neurons Cells, 2021-010(6).
PMID: 34204261 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotLJ Kepser et al. (2021-02-26), Cyclase-associated protein 2 (CAP2) controls MRTF-A localization and SRF activity in mouse embryonic fibroblasts Scientific Reports, 2021-011(1):4789.
PMID: 33637797 -
Species: Human
Sample Types: Whole Cells
Applications: ICCJ Fisher et al. (2020-10-26), Using chemiluminescence imaging of cells (CLIC) for relative protein quantification Sci Rep, 2020-110(1):18280.
PMID: 33106566 -
Species: Rat
Sample Types: Cell Lysates
Applications: Western BlotZ Li et al. (2020-08-12), Uniaxial Static Strain Promotes Osteoblast Proliferation and Bone Matrix Formation in Distraction Osteogenesis In Vitro Biomed Res Int, 2020-02020(0):3906426.
PMID: 32855965 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotE Cienfuegos et al. (2020-07-10), Effect of sodium (S)-2-hydroxyglutarate in male, and succinic acid in female Wistar rats against renal ischemia-reperfusion injury, suggesting a role of the HIF-1 pathway PeerJ, 2020-08(0):e9438.
PMID: 32728491 -
Species: Human
Sample Types: Whole Cells
Applications: ICCG Kaufman et al. (2020-06-26), The self-renewal dental pulp stem cell microtissues challenged by a toxic dental monomer Biosci. Rep., 2020-040(6).
PMID: 32495822 -
Species: Human
Sample Types: Cell Lysates
Applications: Co-ImmunoprecipitationKV Lithgow et al. (2020-04-01), Identification of the Neuroinvasive Pathogen Host Target, LamR, as an Endothelial Receptor for the Treponema pallidum Adhesin Tp0751 mSphere, 2020-05(2).
PMID: 32238570 -
Species: Mouse
Sample Types: Whole Tissue
Applications: Western BlotS Varshney et al. (2019-10-07), A modifier in the 129S2/SvPasCrl genome is responsible for the viability of Notch1[12f/12f] mice BMC Dev. Biol., 2019-119(1):19.
PMID: 31590629 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotK Watanabe et al. (2019-04-24), Leptin enhances cytokine/chemokine production by normal lung fibroblasts by binding to leptin receptor Allergol Int, 2019-00(0).
PMID: 31029506 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotA Sharma et al. (2019-01-09), Metformin exhibited anticancer activity by lowering cellular cholesterol content in breast cancer cells PLoS ONE, 2019-014(1):e0209435.
PMID: 30625181 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotRM Fliefel et al. (2019-01-09), Geranylgeraniol (GGOH) as a Mevalonate Pathway Activator in the Rescue of Bone Cells Treated with Zoledronic Acid: An In Vitro Study Stem Cells Int, 2019-02019(0):4351327.
PMID: 30728841 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotSY Ma'ayeh et al. (2017-12-11), Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells PLoS Negl Trop Dis, 2017-111(12):e0006120.
PMID: 29228011 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotGutknecht M et al. (2015-03-24), The transcription factor MITF is a critical regulator of GPNMB expression in dendritic cells. Cell Commun Signal, 2015-013(0):19.
PMID: 25889792 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotKondo S et al. (2014-02-01), Differential expression of vascular endothelial growth factor in high- and low-metastasis cell lines of salivary gland adenoid cystic carcinoma. Anticancer Res, 2014-034(2):671-7.
PMID: 24510998 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotLiang G et al. (2012-05-15), Normal leptin expression, lower adipogenic ability, decreased leptin receptor and hyposensitivity to Leptin in Adolescent Idiopathic Scoliosis. PLoS ONE, 2012-07(5):e36648.
PMID: 22615788
There are no citations that match your criteria.
Customer Reviews for Human/Mouse/Rat GAPDH Antibody (3)
Customer Images
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Application: Western BlotSample Tested: Xenograft Lysates and Cancer cell lysatesSpecies: Mouse and HumanVerified Customer | Posted 07/03/2018
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Application: Western BlotSample Tested: Human cell lineSpecies: HumanVerified Customer | Posted 03/01/2017Expression of Human p75NGF in several cell lines, with GAPDH levels showing equal loading. Dilution: 1:10,000 in PBS with 5% BSA. Secondary Ab: 1:5,000 anti-mouse IgG.
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Application: Western BlotSample Tested: See PMID 24040198Species: MouseVerified Customer | Posted 02/25/2015
There are no reviews that match your criteria.
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