Human CD3 epsilon APC‑conjugated Antibody

Catalog # FAB100A | R&D Systems, Inc. a Bio-Techne Brand
Clone UCHT1 was used by HLDA to establish CD designation
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FAB100A-025
FAB100A-100
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # UCHT1

Product Summary for Human CD3 epsilon APC‑conjugated Antibody

Immunogen

Human thymocytes (1)

Specificity

Recognizes the epsilon-chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD3 epsilon APC‑conjugated Antibody

Detection of CD3e antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of CD3 epsilon in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD3 epsilon APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood lymphocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD3 epsilon

CD3 epsilon is a key signaling molecule that participates in the formation of the T Cell Receptor (TCR). The alpha beta TCR is composed of six distinct subunits. There is an alpha-beta disulfide-linked heterodimer that recognizes antigen, and this is accompanied by four CD3 subunits that transduce an intracellular signal. CD3 epsilon forms a heterodimer with both CD3 gamma and CD3 delta, creating two signaling complexes. These two heterodimers are supplemented by a CD3 zeta zeta homodimer that lies in the center of the TCR complex, generating a fully-functional TCR (2). 

References

  1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
  2. Call, M.E. and K.W. Wucherpfennig (2005) Annu. Rev. Immunol. 23: 101

Alternate Names

CD3e

Entrez Gene IDs

102133065 (Cynomolgus Monkey); 916 (Human); 12501 (Mouse)

Gene Symbol

CD3E

Product Documents for Human CD3 epsilon APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD3 epsilon APC‑conjugated Antibody

For research use only

Citations for Human CD3 epsilon APC‑conjugated Antibody (5)

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FAQs for Human CD3 epsilon APC‑conjugated Antibody

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  • Q: What is the optimal working concentration of the antibody?

    A: Novus' product datasheets provide a recommended concentration for each validated application that is a starting point for titration. The concentration required will vary from lab to lab due to other variables and thus titration is strongly recommended for optimizing results.

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