CXCL12/SDF-1 alpha Antibody [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29480CL1
![CXCL12/SDF-1 alpha Antibody [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/nbp2-29480cl1_rabbit-cxcl12-sdf-1-alpha-pab-corafluor-1-8920251610102.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Functional
Label
CoraFluor 1
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant human SDF-1alpha
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 24995775)
Specificity
NBP2-29480 recognises human SDF 1 alpha, otherwise known as CXCL12a, a 68 amino acid stromal cell derived CXC chemokine, which arises from alternative splicing of the SDF 1 gene. The two isoforms, SDF 1 alpha and SDF 1 beta, share an identical amino acid sequence, except for an additional four residues in the C terminal region of the beta isoform.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Scientific Data Images for CXCL12/SDF-1 alpha Antibody [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for CXCL12/SDF-1 alpha Antibody [CoraFluor™ 1]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Functional
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: CXCL12/SDF-1 alpha
CXCL12 is a heparin-binding chemokine that regulates lymphopoiesis, enhances the survival of myeloid progenitor cells, regulates the patterning and cell number of neural progenitors, and promotes angiogenesis. CXCL12/SDF-1 alpha is one of several SDF-1 isoforms, all of which can undergo proteolytic processing of the first two N-terminal amino acids by DPPIV/CD26. SDF-1 isoforms signal through CXCR4 and CXCR7 and can also bind Syndecan-4. Their binding to CXCR4 may block the cellular entry of HIV.
Alternate Names
SDF1 alpha, SDF1a
Gene Symbol
CXCL12
Additional CXCL12/SDF-1 alpha Products
Product Documents for CXCL12/SDF-1 alpha Antibody [CoraFluor™ 1]
Product Specific Notices for CXCL12/SDF-1 alpha Antibody [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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