Cy5 Labeled Hyaluronan (Low MW)

Name: Cy5 Labeled Hyaluronan (Low MW)
Brand: R&D Systems
SKU: GL401

R&D Systems, part of Bio-Techne | Catalog # GL401

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Product Summary
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Conjugate

Cy5

Product Summary for Cy5 Labeled Hyaluronan (Low MW)


Formulation	Supplied in 20 mM Tris, pH 8.0
Stability & Storage	• 6 months from date of receipt, -20 to -70 °C as supplied. • 3 months, -20 to -70 °C under sterile conditions after opening. • Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Applications

Studying hyaluronan protein binding.
Used as a substrate for various hyaluronan specific hydrolases and lyases.

Key Features and Benefits:

Excitation at 649 nm and emission at 671 nm.
The fluorescent dye Cy5 is conjugated to the non-reducing end GlcNAc residue through enzymatic conjugation.
Can be separated on 15%-17% SDS-PAGE and directly visualized as a ladder containing 5-100 repeating units (RU) of the HA disaccharide GlcA-β,3-GlcNAc.
Linear response range for Cy5 labeled glycans can be from 10 fmol to 100 pmol, depending on the sensitivity of detection.
Store at < -20 °C. Good for 6 months from date of receipt.

For Details:

Wu et al., (2019) Glycobiology 29:750-754
Wu et al., (2020) Glycobiology 30:970-980

Related Reagents

Click Chemistry

Enzymes and detection reagents

Various Hyaluronidases and Hyaluronan Lyase
HA-binding Proteins
Hyaluronan

Data Examples

Recombinant HYAL1 assay using Cy5 Labeled Hyaluronan (Low MW)/(Cy5-LMW HA) as a substrate
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Recombinant HYAL1 assay using Cy5 Labeled Hyaluronan (Low MW)/(Cy5-LMW HA) as a substrate (Catalog # GL401) was digested with HYAL1 Recombinant Human Hyaluronidase 1/HYAL1 Protein (Catalog # 7358-GH), according to the above protocol and then separated on SDS-gel and visualized with a fluorescent imager. Without digestion, Cy5-LMW HA displays a range of bands from RU5 to about RU100. RU, repeating unit of the HA disaccharide GlcA-β,3-GlcNAc. At high dosage, rhHYAL1 digested all HA species to RU1-RU4 products.

Scientific Data Images for Cy5 Labeled Hyaluronan (Low MW)

Cy5 Labeled Hyaluronan (Low MW).

This substrate can be used for various hyaluronan specific hydrolases and lyases. The fluorescent dye Cy5 is conjugated to the non-reducing end GlcNAc residue. (n, the number of repeating disaccharide units, ranges from about 5 to 100)

Formulation, Preparation, and Storage

Formulation

No Formulation Info in Bulk Inventory

Reconstitution Instructions

Reconstitute at 100 μg/mL in PBS.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Hyaluronan

Hyaluronan, also known as hyaluronic acid (HA) or sodium hyaluronate, is a naturally occurring linear polymer. Hyaluronan is a glycosaminoglycan (GAG) that is ubiquitously present in the extracellular matrix of all vertebrates and is also present in the capsule of some strains of Streptococci. Mammalian hyaluronan is synthesized by one of three distinct hyaluronan synthases (HAS1, 2, and 3), which produce HA polymers with different chain lengths and differ in their rates of synthesis. Whereas high molecular weight HA (> 500 kDa) is anti-angiogenic, anti-inflammatory and immunosuppressive, low molecular weight HA (10 - 500 kDa) is highly angiogenic and pro-inflammatory. HA oligomers are anti-apoptotic and upregulate heat shock protein expression. Functionally, hyaluronan molecules are important for the maintenance of a highly hydrated extracellular matrix in tissues, which is involved in cell adhesion and supports cell migration.

Cy5 Labeled Hyaluronan (Low MW)