Cy5-Fuc Labeled M1N1f
R&D Systems, part of Bio-Techne | Catalog # GL301
Key Product Details
Key Benefits
Learn more about Fluorescent Glycan Labeling and Detection
Conjugate
Cy5
Product Summary for Cy5-Fuc Labeled M1N1f
|
Formula |
Fuc1Man3GlcNAc3Cy5 |
|
Molecular Weight |
2088.96 Da |
|
Formulation |
Supplied in 20 mM Tris, pH 8.0 |
|
Stability & Storage |
• 6 months from date of receipt, -20 to -70 °C |
Applications
- Used as a ligand for lectins and to study glycan protein interaction.
- Used as a substrate for various glycosidases and glycosyltransferases.
- Used as a scaffold for the synthesis of various glycan epitopes such as sialyl Lewis X structures.
Key Features and Benefits:
- Excitation at 649 nm and emission at 671 nm.
- The fluorescent dye Cy5 is conjugated to the C6 position of the core-6 fucose.
- Can be separated on 15%-17% SDS-PAGE and directly visualized as a single band under a fluorescent imager.
- Linear response range for Cy5-labeled glycans can be from 10 fmol to 100 pmol, depending on the sensitivity of detection.
- Store at < -20 °C. Good for 6 months from date of receipt.
For Details:
- Wu et al., (2019) Glycobiology 29:750-754
- Wu et al., (2020) Glycobiology 30:970-980
- A Simple SDS-PAGE-based Technique for Probing Terminal Glycans
Related Reagents
Click Chemistry
- GDP-Cy5-Fucose (ES301)
- CMP-Cy5-Sialic Acid (ES302)
- GDP-Cy3-Fucose (ES401)
- CMP-Cy3-Sialic Acid (ES402)
Enzymes and detection reagents
- Various sialyltransferases
- Various neuraminidases/sialidases
Data Examples
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Cy5-Fuc Labeled M1N1f as a substrate for recombinant MGAT2 assay. Cy5-Fuc labeled M1N1f (M1N1f) (Catalog # GL301) (0.2 pmol per reaction) was treated by a serial dilution of Recombinant Human N-Acetylglucosaminyltransferase2/MGAT2 Protein (Catalog # 10711-GT). The reactions were incubated 30 minutes at 37oC then heat inactivated, and further elongated with Recombinant Human B4GalT1 Protein (Catalog # 3609-GT) at 37oC for 20 minutes. Samples were separated on 15% SDS-PAGE and imaged with TCE staining (top panel) and fluorescent imaging (lower panel). Product 2 is visible with 6.25 ng of MGAT2, and its maximal level was achieved at 100 ng of the enzyme. The conversion of M1N1f to M2f by MGAT2 did not generate obvious mobility shift (data not shown). To overcome this issue, the substrate M1N1f and the product M2f were elongated by B4GalT1 to Product 1 and Product 2, respectively, to create an obvious gel mobility shift. |
Scientific Data Images for Cy5-Fuc Labeled M1N1f
Cy5-Fuc Labeled M1N1f'
This substrate can be used as ligand for lectins and to study various glycosidases and glycosyltransferases. This can also be used as a scaffold for the synthesis of various glycan epitopes such as sialyl Lewis X structures. The fluorescent dye Cy5 is conjugated to the C6 position of the core-6 fucose.Formulation, Preparation, and Storage
Formulation
No Formulation Info in Bulk Inventory
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: M1N1f
Long Name
M1N1f
Additional M1N1f Products
Product Documents for Cy5-Fuc Labeled M1N1f
Product Specific Notices for Cy5-Fuc Labeled M1N1f
For research use only
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